CAM (Complementary and Alternative Medicine) therapies have become increasingly  popular in pediatric populations. phytotherapy are the most common CAM used in children. Yet, little is known about children’s preferences for CAM. But It is expected to become more widely integrated into the modern medical system, including the medical curriculum.The aim of this study is to introduce the prevalenc...

Aim: The aim of this study is to determine the cytotoxic effects Computer Aided Design (CAD) and Manufacturing (CAM) blocks produced by different companies on human peripheral blood lymphocytes MTT assay.
 Methods: Six CAD/CAM materials were investigated: feldspar ceramic VM (Vitablocks Mark II ), resin nano-ceramic LU (Lava Ultimate), hybrid C (Cerasmart), leucite-reinforced LRF (GC LRF),...

A 2-site immunoradiometric assay (IRMA) has been done using Anopheles stephensi fed on Plasmodium vivax blood through parafilm to ascertain at what time in the sporogonic cycle circumsporozoite (CS) antigen can be detected, and to detect CS antigen in mosquitoes squashed on filter paper (FP) and cellulose acetate membrane (CAM). The CS antigen was detectable only in mature oocysts, a day prior ...

Calcium/calmodulin-dependent kinase kinase 2 (CaMKK2) has been implicated in a range of conditions and pathologies from prostate to hepatic cancer. Here, we describe the expression in Escherichia coli and the purification protocol for the following constructs: full-length CaMKK2 in complex with CaM, CaMKK2 'apo', CaMKK2 (165-501) in complex with CaM, and the CaMKK2 F267G mutant. The protocols d...

An in vivo assay for phospholipase D (PLD; EC 3.1.4.4) activity, based on its transphosphatidylation property, is described in detail and was used to study putative post-translational regulation mechanisms of PLD activity in carnation [Dianthus caryophyllus L.) petals. A variety of agents was applied to petal discs. The calmodulin (CaM) antagonists propranolol, /V-(6-aminohexyl)-5-chloro-1 -nap...

Because cancer is often treated with combination therapy, unexpected pharmacological effects can occur because of drug–drug interactions. Several drugs are able to cause upregulation or downregulation of drug transporters or cytochrome P450 enzymes, particularly CYP3A4. Induction of CYP3A4 may result in decreased plasma levels and therapeutic efficacy of anticancer drugs. Since the pregnane X r...

For virtually all cilia and eukaryotic flagella, the second messengers calcium and cyclic adenosine monophosphate are implicated in modulating dynein- driven microtubule sliding to regulate beating. Calmodulin (CaM) localizes to the axoneme and is a key calcium sensor involved in regulating motility. Using immunoprecipitation and mass spectrometry, we identify members of a CaM-containing comple...

BACKGROUND Autophosphorylation of the Ca(2+)/calmodulin (CaM)-dependent protein kinase II (CaMKII) at T286 generates partially Ca(2+)/CaM-independent "autonomous" activity, which is thought to be required for long-term potentiation (LTP), a form of synaptic plasticity thought to underlie learning and memory. A requirement for T286 autophosphorylation also for efficient Ca(2+)/CaM-stimulated CaM...

an increasing number of asthmatic patients are attracted by complementary and and alternative medicine (cam). the aim of this study was to estimate the prevalence and describe the characteristics of cam use by children with asthma in a paediatric allergy clinic in istanbul, turkey.the parents of asthmatic children were invited to participate in a cross-sectional survey study. current asthma tre...

Infectious Laryngotracheitis (ILT) is an acute highly contagious respiratory disease of chickens. It has significant economic importance due to mortalities and the decrease in egg production. In this study, twenty nine samples from different layer farms were collected outbreaks that occurred waset province, Iraq, at period November 2021 till March 2022 detect ILTV by molecular test through real...