The human multidrug resistance P-glycoprotein uses ATP to transport a wide variety of structurally unrelated cytotoxic compounds out of the cell. In this study, we used cysteine-scanning mutagenesis and cross-linking studies to identify residues that are exposed to the drug-binding site upon vanadate trapping. In the absence of nucleotides, C222(TM4) was cross-linked to C868(TM10) and C872(TM10...

The sliding β-clamp, an important component of the DNA replication and repair machinery, is drawing increasing attention as a therapeutic target. We report the crystal structure of the M. tuberculosis β-clamp (Mtbβ-clamp) to 3.0 Å resolution. The protein crystallized in the space group C222(1) with cell-dimensions a = 72.7, b = 234.9 & c = 125.1 Å respectively. Mtbβ-clamp is a dimer, and exhibi...

The donor-acceptor complexes PPh4[Cl(succinimide)2] and PPh4[Cl(N-chlorosuccinimide)2] have been prepared by reactions of PPh4Cl with succinimide and N-chlorosuccinimide, re­ spectively. in acetonitrile solutions. The complexes have been characterized by IR spectros­ copy and by crystal structure determinations. The crystal structure of N-chlorophthalimide has also been solved. N-chlorophthalim...

BACKGROUND Rhodopsin, the prototypic member of G protein-coupled receptors (GPCRs), undergoes isomerization of 11-cis-retinal to all-trans-retinal upon photoactivation. Although the basic mechanism by which rhodopsin is activated is well understood, the roles of whole transmembrane (TM) helix-III during rhodopsin photoactivation in detail are not completely clear. PRINCIPAL FINDINGS We herein...

Kuramochi, Yukio, Chee Chew Lim, Xinxin Guo, Wilson S. Colucci, Ronglih Liao, and Douglas B. Sawyer. Myocyte contractile activity modulates norepinephrine cytotoxicity and survival effects of neuregulin-1 . Am J Physiol Cell Physiol 286: C222–C229, 2004. First published October 1, 2003; 10.1152/ajpcell.00312.2003.—The purpose of this study is to test the hypothesis that mechanical and electrica...

The high-potential iron-sulfur protein (iso-form II) isolated from Ectothiorhodospira vacuolata has been crystallized and its three-dimensional structure determined by molecular replacement procedures and refined to 1.8-A resolution with a crystallographic R factor of 16.3%. Crystals employed in the investigation belonged to the space group C222(1) with unit cell dimensions of a = 58.4 A, b = 6...

Demand for mineral resources is increasing, necessitating exploitation of lower grade and more heterogeneous orebodies. The high variability inherent in such orebodies leads to an increase the cost, complexity environmental footprint associated with mining processing. Enhanced knowledge orebody characteristics thus vital companies optimize profitability. We present a pilot study investigate pre...

The high-potential iron-sulfur protein (iso-form 11) isolated from Ectothiorhodospira vacuolata has been crystallized and its three-dimensional structure determined by molecular replacement procedures and refined to 1.8-A resolution with a crystallographic R factor of 16.3%. Crystals employed in the investigation belonged to the space group C222, with unit cell dimensions of a = 58.4 A, b = 64....

| References | Full Text: HTML, PDF (Size: 554K) Save Article Study of the Biosynthesis of 1-Octen-3-ol Using a Crude Homogenate of Agaricus bisporus in a Bioreactor (p C135-C139) R.O. Morawicki, R.B. Beelman Published Online: Feb 6 2008 12:00AM DOI: 10.1111/j.1750-3841.2007.00660.x Abstract | References | Full Text: HTML, PDF (Size: 508K) Save Article Properties of High-Oleic Palm Oils Derived...