Five well characterised strains of Clostridium difficile of differing virulence and two Escherichia coli strains, a verotoxigenic O157:H7 isolate and a urinary isolate, were examined for cell surface hydrophobicity and charge, and haemagglutinating ability. Phase partition in hexadecane or octan-1-ol was similar for C. difficile and E. coli, as was retention by hydrophobic interaction chromatog...

While both Campylobacter coli and C. jejuni are frequent causes of human campylobacteriosis, a common conclusion of health care professionals and researchers alike is that C. jejuni is the more pathogenic or virulent species. C. jejuni has been shown to be superior in enterotoxin production, suggesting that C. jejuni is more enterotoxigenic, and as such, potentially more virulent than C. coli. ...

Enterohemorrhagic Escherichia coli O157:H7 infection causes several hundred cases of food poisoning every year in Japan. In severe cases, this type of food poisoning can be fatal. In the present study, we examined the induction of HSP70 in E. coli O157:H7 cells at various temperatures and the thermotolerance of E. coli O157:H7 cells alone and in contaminated food following pre-heating. We evalu...

Polynucleotide relationships were examined among many representatives of the Enterobacteriaceae by means of agar, membrane filter, and hydroxyapatite procedures. The amount of deoxyribonucleic acid (DNA) that reassociated was dependent, especially in interspecific reactions, on the annealing temperature. In only three cases: Escherichia coli-Shigella flexneri, Salmonella typhimurium-S. typhi, a...

Background: Subunit vaccines are appropriate vaccine candidates for the prevention of some infections. In this study, three immunogenic proteins of Mycobacterium tuberculosis, including HspX, Ppe44, and EsxV as a new construction, were expressed alone and as a fusion protein to develop a new vaccine candidate against tuberculosis infection. Methods: To make the fusion protein, the three genes ...

In this Research work, Silver Nanoparticles were green synthesized from Psidium guajava leaves and different Characterization techniques including UV-Visible, FT-IR, SEM XRD all employed to ascertain the absorption peaks, functional group, surface morphology crystalline size of nanoparticles respectively. These applied against four pathogens namely, S. aureus (gram- positive bacteria), E. coli ...

A temperate phage designated obeta1 (omicron beta) was mitomycin C induced and isolated from heat-labile enterotoxin (LT)-producing Escherichia coli E2631-C2. Phage obeta1 infected the nonlysogenic, nontoxigenic, mitomycin C-sensitive strain of E. coli K-12 (CSH38) and converted it to lysogeny and enterotoxigenicity. After the establishment of lysogeny, E. coli CSH38(obeta1) produced produced L...

Genes encoding superoxide dismutase (SOD: EC 1.15.1.1) were cloned from Campylobacter jejuni NCTC 11351 and Campylobacter coli UA585 by heterologous complementation of a SOD-deficient Escherichia coli mutant. Deletion analysis of the cloned C. jejuni DNA assigned the sod gene to a 1.2 kb insert and this contained an open reading frame of 660 bp. The deduced gene product of 220 amino acids was 7...

Folates are cofactors for biosynthetic enzymes in all eukaryotic and prokaryotic cells. Animals cannot synthesize folate and must acquire it from their diet or microbiota. Previously, we showed that inhibiting E. coli folate synthesis increases C. elegans lifespan. Here, we show that restriction or supplementation of C. elegans folate does not influence lifespan. Thus, folate is required in E. ...

A deposition technology of amorphous carbon coating (a-C), doped with copper (C-$$$Cu), has been developed. The C-Cu was performed the simultaneous operation arc- and pulse-arc sources graphite cathodes, respectively. There studied antibacterial activity a-C against E. coli, S. aureus P. aeruginosa. copper-doped bactericidal number bacterial colonies is five times lower in compare Ti.