anthrax is one of the important zoonose disease between human and cattle. bacillus anthracis is a gram- positive bacterium which its tripartite protein toxin contains protective antigen (pa), edema factor (ef) and lethal factor (lf) and a poly d- glutamic acid capsule. a recent resurgence of interest in b.anthracis resulted in improved methods for production, separation and purification of the ...

125I-labelled heat-labile toxin (from Escherichia coli) and 125I-labelled cholera toxin bound to immobilized ganglioside GM1 and Balb/c 3T3 cell membranes with identical specificities, i.e. each toxin inhibited binding of the other. Binding of both toxins to Balb/c 3T3 cell membranes was saturable, with 50% of maximal binding occurring at 0.3 nM for cholera toxin and 1.1 nM for heat-labile toxi...

objective: aflatoxin is important in the food industry, in animal husbandry and the medical area; there are enormous negative economic impacts due to this toxin. numerous studies have researched extracts and plant compounds with the intent to reduce the growth of aflatoxin-producing organisms, inhibit toxin production and suppress the major toxin encoded genes (i.e., aflr) in these organisms. l...

methods campylobacter jejuni atcc 33291 and seven isolates donated from instituto de biotecnologia were used in this study. the presence of cdt genes in c. jejuni strains was determined by pcr. to evaluate the effect of cdt, hela cells were treated with bacterial lysate, and the damage and morphological changes were analyzed by microscopy, immunofluorescence staining, and flow cytometry. to eva...

An inter-laboratory collaborative study was performed to evaluate the performance of Biochip Array Technology (BAT) Myco 7 method. The is a method which simultaneously and quantitatively detects 20 mycotoxins including aflatoxins B 1 , 2 G ochratoxin A, deoxynivalenol, zearalenone, fumonisin 3 T-2 HT-2 toxin. BAT collaboratively evaluated by nine government private Association American Feed Con...

The solubility of the homogeneous, nonenzymatic structural protein isolated and purified from bovine heart mitochondria when incubated with the murine toxin of Pasteurella pesfis at pH 11.0 increases as the concentration of the toxin is increased. This indicates that a protein-protein interaction occurs between the monomeric units of the structural protein and the toxin. This interaction result...

The solubility of the homogeneous, nonenzymatic structural protein isolated and purified from bovine heart mitochondria when incubated with the murine toxin of Pasteurella pesfis at pH 11.0 increases as the concentration of the toxin is increased. This indicates that a protein-protein interaction occurs between the monomeric units of the structural protein and the toxin. This interaction result...

Botulinum toxin, the causal agent of botulism, is employed in therapeutic settings for a number purposes. toxin injections address aesthetic issues induced or worsened by muscle contraction controlling local neurotransmission. Therapy has transient impact; recovery generally clinically visible few months following treatment. Improper injection placement diffusion to unintended locations might r...

background: sequence variations in glycoproteins of influenza virus surface impel us to design new candidate vaccines yearly. ectodomain of influenza m2 protein is a surface and highly conserved protein. m2e in influenza vaccines may eliminate the need for changing vaccine formulation every year. objectives: in this study, a recombinant baculovirus containing m2e and cholera toxin subunit b fus...