Lee YJ, Suh HN, Han HJ. Effect of BSA-induced ER stress on SGLT protein expression levels and -MG uptake in renal proximal tubule cells. Am J Physiol Renal Physiol 296: F1405–F1416, 2009. First published February 11, 2009; doi:10.1152/ajprenal.90652.2008.—Recent studies demonstrated that endoplasmic reticulum (ER) stress regulates glucose homeostasis and that ER stress preconditioning which ind...

Immunological tolerance produced in rabbits by neonatal injections of BSA can be terminated by a series of injections of certain heterologous serum albumins which cross-react with BSA. Injections of albumins distantly related to BSA were more effective in terminating the tolerant state than injections of albumins closely related to BSA. It was concluded from results obtained with several hetero...

BACKGROUND The stability of protein drugs remains one of the key hurdles to their success in the market. The aim of the present study was to design a novel nanoemulsion drug-delivery system (NEDDS) that would encapsulate a standard-model protein drug--bovine serum albumin (BSA)--to improve drug stability. METHODS The BSA NEDDS was prepared using a phase-inversion method and pseudoternary phas...

The prevalence of cancer in Indonesia has increased by 8.8 percent the last two years (2018 and 2020) terms adding new cases mortality. Because therefor treatment becomes important reducing high number additional mortality due to patients. Intensive is surgery, chemotherapy, radiotherapy immunotherapy. Chemotherapy drugs used for include herbal plant-based (Cancer Fit) cisplatin its buy from on...

Tyrosine was explored as a cross-linking agent to form cross-linked bovine serum albumin (BSA) using laccase catalyst. Liquid chromatography-mass spectrometry (LC-MS) and fluorescence spectra indicated that tyrosine can be mainly oxidized dityrosine. Spectra analysis molecular weight were used characterize the BSA treated with laccase. Both SDS-PAGE size exclusion chromatography confirmed forma...

The aim of this study was to label Bovine Serum Albumin (BSA) by Rhodamine B Isothiocyanate (RBITC) and test its stability in vivo. RBITC-BSA was labelled by the improved Marshall’s method, scanned at full wavelength, and the RBITC:BSA ratio was calculated. Blood and urine samples were obtained from rabbits injected with the RBITC-BSA complex and SDS-PAGE analysis was performed. Full wavelength...

For reducing protein aggregation in foam fractionation, the role of pH-induced structural change in the interface-induced protein aggregation was analyzed using bovine serum albumin (BSA) as a model protein. The results show that the decrease in pH from 7.0 to 3.0 gradually unfolded the BSA structure to increase the molecular size and the relative content of β-sheet and thus reduced the stabili...

Interaction mechanism of cefoperazone sodium (CFP) and ofloxacin (OFX) with bovine serum albumin (BSA) at different temperatures by fluorescence spectrometry method was studied. Results showed that CFP or OFX could quench the fluorescence of BSA. The fluorescence would quench to a larger degree when OFX (or CFP) was added to the system of BSA-CFP (or BSAOFX). The quenching mechanism of the comb...

The pulsed electric fields (PEF) treatment is a novel method for obtaining glycated proteins by way of a Maillard reaction between proteins and polysaccharides but its effect on the preparation of protein-monosaccharide conjugate has not been explored. This study aimed to prepare bovine serum albumin (BSA)-glucose and BSA-mannose conjugates using PEF in pH 10.0 at an intensity of 10 or 20 kV/cm...