We designed a protein ladder (hereafter referred to as "Mega-tag") that contains 14 of the most commonly used epitope tags fused to molecular weight markers. The Mega-tag ladder can be simultaneously visualized when anti-tag antibodies are used to detect epitope-tagged recombinant proteins in Western blots. The logarithm of molecular weights and relative mobility of the Mega-tag protein ladder ...

After 20 years of a static clinical ladder program at our institution, the clinical ladder program was completely redesigned using a staff nurse-led shared governance structure to re-envision the program as an innovative, staff leadership model to meet our organizational nursing mission and vision strategic plans around retention and professional development. The literature demonstrated a lack ...

C = ladder network stage capacitance E = difference operator L = ladder network stage inductance ‘ = number of ladder network stages m = voltage measurement node n = ladder network node identifier, 0 n ‘ Rt = termination resistance s = Laplace variable V t = transmission line driving signal ~ Vn = Laplace transform of the voltage at any node n x = length of transmission line min = smallest wave...

Absrracr-The low sensitivity property of lossless discrete integrator (LDI) low-pass ladder filters is shown to be preserved in lossless discrete differentiator (LDD) high-pass ladder filters 111. The exact design method for LDI ladder filters given in [Z] is further developed by introducing a set of closed-form design formulas for digital ah-pole Chebyshev transfer functions. A new technique f...

3 B assessment and the development of appropriate intervention strategies for a particular client require consideration of the individual in relation to a larger social context. To accomplish this, we use principles and concepts derived from systems theory. Systems theory is a way of elaborating increasingly complex systems across a continuum that encompasses the person-inenvironment (Anderson,...

On a summer afternoon in the late 1990s in New York City’s Tompkins Square Park, sociologist Bruce Western and his colleague Katherine Beckett engaged in a conversation that would change the course of their research. At the time, Western studied the social programs of European welfare states. Against the backdrop of a park that had seen a surge in drug dealing and homeless encampment in the 198...

Two new genera of crinoid-associated pontoniine shrimps, Brucecaris, new genus, and Unguicaris, new genus, are described and illustrated. Brucecaris is established for Periclimenes tenuis Bruce. Unguicaris panglaonis, new genus, new species, is described from crinoid-associated material collected at Panglao Island, Bohol Sea, The Philippines. Three species, Periclimenes alegrias Bruce, Periclim...

The remarks on Bruce Smith’s work, that follow, have been presented at the Bruce D. Smith Memorial Conference held by the Department of Economics of the University of Texas at Austin, onApril 12–13, 2003. The conference was organized by Scott Freeman, Sebastian Centennial Professor in the Department of Economics of the University of Texas at Austin, and Bruce Champ, of the Research Department o...