نتایج جستجو برای: bradyporus latipes

تعداد نتایج: 1249  

Journal: :Zoological science 2010
Katsueki Ogiwara Takashi Ikeda Takayuki Takahashi

We sought to establish a new in vitro ovulation model using the whole ovaries of the medaka. Ovaries of the fish, which had been acclimated to the usual reproductive conditions (26 degrees C, 14 h light/10 h dark) and which had then been kept at least one day at 30 degrees C, were isolated 2 h before the expected in vivo ovulation time. When the ovaries were cultured in 90% medium 199 solution ...

Journal: :Chemical & pharmaceutical bulletin 2012
Matsutake Higa Megumi Imamura Kazuhito Ogihara Toshimasa Suzuka

Five new flavonoids, 5,8-dihydroxy-3,7-dimethoxy-3',4'-methylenedioxyflavone (1), 7-hydroxy-3,5-dimethoxy-3',4'-methylenedioxyflavone (2), 7-(2,3-dihydroxy-3-methylbutoxy)-3,5-dimethoxy-3',4'-methylenedioxyflavone (3), 7-(2,3-dihydroxy-3-methylbutoxy)-3,3',4',5-tetramethoxyflavone (4), and 7-(2,3-dihydroxy-3-methylbutoxy)-3,3',4',5,8-pentamethoxyflavone (5), were isolated from the leaves of Mel...

Journal: :Science 2006
Martina Rembold Felix Loosli Richard J Adams Joachim Wittbrodt

The cellular mechanisms underlying organ formation are largely unknown. We visualized early vertebrate eye morphogenesis at single-cell resolution by in vivo imaging in medaka (Oryzias latipes). Before optic vesicle evagination, retinal progenitor cells (RPCs) modulate their convergence in a fate-specific manner. Presumptive forebrain cells converge toward the midline, whereas medial RPCs remai...

Journal: :Italian journal of anatomy and embryology = Archivio italiano di anatomia ed embriologia 2008
Gen Niimi Hideki Imada

The medaka, Oryzias latipes is a useful animal model for the study of primary vasculature in vertebrate embryos. Using benzidine stain for erythroid cells, we found presumptive erythropoietic foci in the yolk sac vitellolysis zone at stage 39. These foci were present in the yolk syncytial layer, in the extravascular and vitellolysis zone from 9 days post fertilization (dpf) to 11 dpf, and then ...

Journal: :Briefings in functional genomics & proteomics 2008
Daisuke Kobayashi Hiroyuki Takeda

The medaka Oryzias latipes is a small egg-laying freshwater teleost, and has become an excellent model system for ecotoxicology, developmental genetics, genomics and evolutionary biology studies. A high-quality draft genome sequence (700 Mb) of the medaka inbred strain, Hd-rR, has now been completed. In this review, we present an overview of the medaka genome project and describe the substantia...

Journal: :Toxicological sciences : an official journal of the Society of Toxicology 2003
Duane B Huggett Christy M Foran Bryan W Brooks Jim Weston Bethany Peterson K Erica Marsh Thomas W La Point Daniel Schlenk

Attempts to better understand causal factors affecting estrogenicity in municipal wastewater have primarily focused on analytical evaluation of specific chemical estrogens and the use of estrogen receptor (ER) based in vitro assays. To compare analytical,in vitro, and in vivo assays for estrogenicity, wastewater from four New York and one Texas municipal wastewater facilities was evaluated for ...

Journal: :Mechanisms of Development 2004
Tomomi Watanabe Satoshi Asaka Daiju Kitagawa Kota Saito Ryumei Kurashige Takao Sasado Chikako Morinaga Hiroshi Suwa Katsutoshi Niwa Thorsten Henrich Yukihiro Hirose Akihito Yasuoka Hiroki Yoda Tomonori Deguchi Norimasa Iwanami Sanae Kunimatsu Masakazu Osakada Felix Loosli Rebecca Quiring Matthias Carl Clemens Grabher Sylke Winkler Filippo Del Bene Joachim Wittbrodt Keiko Abe Yousuke Takahama Katsuhito Takahashi Toshiaki Katada Hiroshi Nishina Hisato Kondoh Makoto Furutani-Seiki

We report here mutations affecting various aspects of liver development and function identified by multiple assays in a systematic mutagenesis screen in Medaka. The 22 identified recessive mutations assigned to 19 complementation groups fell into five phenotypic groups. Group 1, showing defective liver morphogenesis, comprises mutations in four genes, which may be involved in the regulation of ...

Journal: :Mechanisms of Development 2004
Makoto Furutani-Seiki Takao Sasado Chikako Morinaga Hiroshi Suwa Katsutoshi Niwa Hiroki Yoda Tomonori Deguchi Yukihiro Hirose Akihito Yasuoka Thorsten Henrich Tomomi Watanabe Norimasa Iwanami Daiju Kitagawa Kota Saito Satoshi Asaka Masakazu Osakada Sanae Kunimatsu Akihiro Momoi Harun Elmasri Christoph Winkler Mirana Ramialison Felix Loosli Rebecca Quiring Matthias Carl Clemens Grabher Sylke Winkler Filippo Del Bene Ai Shinomiya Yasuko Kota Toshiyuki Yamanaka Yasuko Okamoto Katsuhito Takahashi Takeshi Todo Keiko Abe Yousuke Takahama Minoru Tanaka Hiroshi Mitani Toshiaki Katada Hiroshi Nishina Noboru Nakajima Joachim Wittbrodt Hisato Kondoh

A large-scale mutagenesis screen was performed in Medaka to identify genes acting in diverse developmental processes. Mutations were identified in homozygous F3 progeny derived from ENU-treated founder males. In addition to the morphological inspection of live embryos, other approaches were used to detect abnormalities in organogenesis and in specific cellular processes, including germ cell mig...

Journal: :Journal of embryology and experimental morphology 1972
N Satoh N Egami

Mitotic and meiotic activities of germ cells during early development in the medaka, Oryzias latipes, are dealt with in this report. Primordial germ cells were obviously distinguishable from somatic cells 3 days after fertilization and began to proliferate about 7 days after fertilization. The mean number of primordial germ cells increased during a period of 7-10 days after fertilization, reach...

ژورنال: آبزیان زینتی 2015
بهمنی, محمود, سلامات, نگین, سوداگر, محمد, مازندرانی, محمد, نوروزفشخامی, محمدرضا, یزدانی ساداتی, محمدعلی,

کشت بافت،کاربردهای آن و استفاده از این روش برای تکثیر سلول‌های فولیکولی تخمک توضیح داده شد. مواد و روش کار مورد نیاز برای جداسازی و کشت سلول‌های فولیکولی تاسماهی استرلیاد در محیط کشت L-15 حاوی سرم گوساله جنینی (FBS) و آنتی‌بیوتیک‌ها، بررسی تکثیر آن‌ها و ترشح هورمون‌های استروییدی بیان گردید. طبق نتایج کسب‌ شده سلول‌های فولیکولی کشت داده شده، تکثیر شدند و هورمون‌های تستوسترون (T)، 17- بتا استر...

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