Bone tissue engineering provides many advantages for repairing skeletal defects. Although many different kinds of biomaterials have been used for bone tissue engineering, safety issues must be considered when using them in a clinical setting. In this study, we examined the effects of using a common clinical item, a hemostatic gelatin sponge, as a scaffold for bone tissue engineering. The use of...

To establish a gold/silica hybrid, nanomaterials (Au/SiO2) were incorporated into gelatin methacrylate/chitosan matrix. By using FESEM, compressive strength testing, and conductivity/resistance measurements on (G)/chitosan (C), G/C-Au@SiO2 hydrogels developed. Biocompatibility investigations osteoblasts MG-63 cells carried out to determine whether the cell was compatible with conductive hydroge...

Hybrid scaffolds based on bioactive glass (BAG) particles (<38 µm), covalently linked to gelatin (G*) using 3-glycidoxypropyltrimethoxysilane (GPTMS), have been studied for bone bioengineering. In this study, two compositions (13-93 and 13-93B20 (where 20% of the SiO2 was replaced with B2O3)) were introduced in matrix. The Cfactor (gelatin/GPTMS molar ratio) kept constant at 500. hybrids obt...

In this article we investigate the possibility of scaling down enzyme-gelatin modified electrodes by spin coating the enzyme-gelatin layer. Special attention is given to the electrochemical behavior of the selected enzymes inside the gelatin matrix. A glassy carbon electrode was used as a substrate to immobilize, in the first instance, horse heart cytochrome c (HHC) in a gelatin matrix. Both a ...

This work reports on the preparation and characterization of natural composite materials prepared from bacterial cellulose (BC) incorporated into a gelatin matrix. Composite morphology was studied using scanning electron microscopy and 2D Raman imaging revealing an inhomogeneous dispersion of BC within the gelatin matrix. The composite materials showed controllable degrees of transparency to vi...

Background: engineering new bone tissue with cells and a synthetic extracellular matrix represents a new approach for the regeneration of mineralized tissues compared with the transplantation of bone (autografts or allografts). Methods: in this study, to mimic the mineral and organic component of natural bone, hydroxapatite (HA) and gelatin (GEL) composite scaffolds were prepared. The raw mater...

We previously reported that a macrophage response that increased binding to 125I-radiolabeled soluble denatured collagen (gelatin) was induced by preincubation of macrophage with a 70-kDa amino-terminal fibronectin fragment and soluble nonlabeled gelatin [S. F. Penc, F. A. Blumenstock, J. E. Kaplan (1995) J. Leukoc. Biol. 58, 501-509]. We now report that neither protein synthesis nor recycling ...

The performance of biomaterials-based therapies can be hindered by complications associated with surgical implant, motivating the development of materials systems that allow minimally invasive introduction into the host. In this study, we created cell-adhesive and degradable gelatin scaffolds that could be injected through a conventional needle while maintaining a predefined geometry and archit...

demineralized bone matrix has been successfully commercialized as an alternative bone graft material that not only can function as filler but also as an osteoinductive graft. numerous studies have confirmed its beneficial use in clinical practice. heterotopic ossification after internal fixation combined with the use of demineralized bone matrix has not been widely reported. in this paper we de...