BACKGROUND Shake flasks are widely used during the development of bioprocesses for recombinant proteins. Cultures of recombinant Escherichia coli with orbital mixing (OM) have an oxygen limitation negatively affecting biomass growth and recombinant-protein production. With the aim to improve mixing and aeration in shake flask cultures, we analyzed cultures subjected to OM and the novel resonant...

Microalgal growth curve, after the exponential phase, shows a stationary phase where algalbiomass production is inhibited and remains constant by some factors such as nutrientdepletion and intrinsic behavior. The present study is concerned with evaluating effects ofthe culture medium condition and intrinsic behavior on biomass production by Dunaliellatertiolecta. To this end, effect of pH, nutr...

—The effect of floods and base flow on temporal variation in algal biomass on small smooth streambed stones and creviced sand-cases of the caddisfly (Insecta: Trichoptera) Gumaga nigricula was examined in Big Sulphur Creek in coastal Northern California. Replicates of stones and cases were sampled 34 times over a 14 mo period that included nine floods. Stones had ;23 the algal biomass of cases,...

Tropospheric O3 reduces growth and yield of many crop species, whereas CO2 ameliorates the negative effects of O3. Thus, in a combined elevated CO2 and O3 atmosphere, seed yield is at least restored to that of charcoal-filtered (CF) air at ambient CO2. The CO2induced yield increase in CF air is highly variable, suggesting other potential resource limitations. To understand such variability in r...

Lignocellulosic biomass composes a diversity of feedstock raw materials representing an abundant and renewable carbon source. In majority lignocellulose is constituted by carbohydrate macromolecules, namely cellulose and hemicellulose, and by lignin, a polyphenilpropanoid macromolecule. Between these biomacromolecules, there are several covalent and non-covalent interactions defining an intrica...

Limitation of marine primary production by the availability of nitrogen or phosphorus is common. Emiliania huxleyi, a ubiquitous phytoplankter that plays key roles in primary production, calcium carbonate precipitation and production of dimethyl sulfide, often blooms in mid-latitude at the beginning of summer when inorganic nutrient concentrations are low. To understand physiological mechanisms...

The purpose of this study was to investigate what older adults with severe, moderate, or no mobility limitation consider motives for and barriers to engaging in physical exercise. Community-dwelling adults (N=645) age 75-81 years completed a questionnaire about their motives for and barriers to physical exercise and answered interview questions on mobility limitation. Those with severely limite...

PURPOSE to examine the combined effect of cohabitation status and social participation, respectively, and socioeconomic position on onset of mobility limitations in a prospective study among older Danes. DESIGN AND METHODS logistic regression analyses with combined exposure variables were performed in a study population of 2,839 older men and women from the Danish Intervention Study on Preven...

Boron (B) is essential in plants but often present at low concentrations in the environment. To investigate how plants survive under conditions of B limitation, we conducted a transcriptome analysis and identified NIP5;1, a member of the major intrinsic protein family, as a gene upregulated in B-deficient roots of Arabidopsis thaliana. Promoter-beta-glucuronidase fusions indicated that NIP5;1 i...