The Water Framework Directive 2000/60/EC (WFD) requires Member States of the European Union (EU) to use a pool bioindicators, that is, Biological Quality Elements (BQEs), including fish species and each State shall, in fact, certify compliance with ecological quality goals WFD. New Italian Index Ecological Fish Communities (NISECI) is WFD-compliant index for analysis BQE river water bodies, bot...