Direct electrochemical electron transfer (DET) of oxidoreductases has attracted increasing attention in pure and applied bioelectrochemistry (e.g. biosensors biofuel cells) over the last decades. We report here a systematic study DET-type bioelectrocatalysis membrane-bound redox enzyme fructose dehydrogenase (FDH, Gluconobacter sp.), on variable-length variably terminated thiol self-assembled m...

The fabrication of bioelectrocatalytic protein electrodes by the simple compression carbon nanotube and powders was investigated using a series proteins including bilirubin oxidase (BOx), bovine serum albumin (BSA), catalase, cytochrome C (Cyt C), diaphorase, FAD-dependent glucose dehydrogenase (FAD-GDH), galactose (GAOx), (GOx), horseradish peroxidase (HRP), laccase urease. isoelectric points ...

The ability of Fe(III)protoporphyrin(IX) chloride (hemin), microperoxidase-11 (MP-11), and cytochrome c (cyt-c) to oxidize 2-methoxyphenol (guaiacol) was examined in the room-temperature ionic liquids (IL) 1-butyl-3-methylimidazolium bis(trifluoromethylsulfonyl)imide ([bmim][Tf2N]) and the hexafluorophosphates of 1-butyland 1-octyl-3-methylimidazolium, ([bmim][PF6] and [omim][PF6]), respectivel...

Substrate specificity in the aldo-keto reductase (AKR) superfamily is determined by three mobile loops positioned at the top of the canonical (α/β)(8)-barrel structure. These loops have previously been demonstrated to be modular in a well-studied class of AKRs, in that exchanging loops between two similar hydroxysteroid dehydrogenases resulted in a complete alteration of substrate specificity (...