Expression of eukaryotic proteins in E. coli often results in their aggregation. Proper folding and solubility of therapeutical proteins are the pre-requisite for their bioactivity. This is not achieved in cytoplasmic expression in E. coli because of the absence of disulfide bonds formation. A novel expression/secretion vector was constructed which exploited β-lactamase signal sequence to trans...

ChEMBL is an open large-scale bioactivity database (https://www.ebi.ac.uk/chembl), previously described in the 2012 and 2014 Nucleic Acids Research Database Issues. Since then, alongside the continued extraction of data from the medicinal chemistry literature, new sources of bioactivity data have also been added to the database. These include: deposited data sets from neglected disease screenin...

Effects of alloying element Ca on the corrosion behavior and bioactivity of films formed by plasma electrolytic oxidation (PEO) on AM60 alloys were investigated. The corrosion behavior was studied by conducting electrochemical tests in 0.9% NaCl solution while the bioactivity was evaluated by soaking the specimens in simulated body fluid (SBF). Under identical anodization conditions, the PEO fi...

Recombinant human erythropoietin (rHuEPO) variants have been constructed to identify amino acid residues important for biological activity. Immunoassays were used to determine the effect of each mutation on rHuEPO folding. With this strategy, we could distinguish between mutations that affected bioactivity directly and those that affected bioactivity because the mutation altered rHuEPO conforma...

Potassium (2R,3R)-2,3,4-trihydroxy-2-methylbutanoate (1) was identified as a leaf-closing substance in the nyctinastic plant, Leucaena leucocephala. Compound 1 showed strong leaf-closing activity toward L. leucocephala and was not effective against other nyctinastic plants. The potassium ion was indispensable for the bioactivity of 1. Compound 1 gradually lost its bioactivity because of the exc...

objective(s): cao–mgo–sio2 system bioceramics possess good characteristics for hard tissue engineering applications. the aim of the study was to synthesize the nano powder by using a sol-gel method and evaluate of bioactivity in the cells culture. methods: to characterize of powder x-ray diffraction (xrd), transmission electron microscopy (tem) and to evaluate the bioactivity sample cell seedin...

objective(s): so far, extensive research has been conducted on the preparation and characterization of nano ceramics based on ca-si by sol- gel method and bioactivity was evaluated, but, a few researches have paid attention to the preparation of materials by mechanical activation (ma). the aim of this study was the preparation of akermanite nano powder by mechanical activation method and bioact...

Escalation in number of resistant pathogens and diseases enhanced scientists to explore the unconventional habitats for bioactive compounds. Marine microorganisms represent a promising source for natural products due to the incredible diversity of chemical compounds that were isolated. Due to distinctive features and biodiversity of Red Sea biota, it was selected as a resource for isolation of ...

Titanium (Ti) and Ti-based alloy are widely used in the biomedical field owing to their excellent mechanical compatibility and biocompatibility. However, the bioinert bioactivity and biotribological properties of titanium limit its clinical application in implants. In order to improve the biocompatibility of titanium, we modified its surface with TiOx/TiNx duplex composite films using a new met...