Agrobacterium vitis, the causal agent of grape crown gall, can have severe economic effects on grape production. The bacterium survives systemically in vines and, therefore, is disseminated in propagation material. We developed an assay for use in indexing programs that is efficient and sensitive for detecting A. vitis in grape tissue. Initially, real-time polymerase chain reaction (PCR) primer...

Need for Improved Methods to Validate, Diagnose, and Monitor Copy Number Variations Given the high incidence and clinical impact of CNVs, a precise, rapid, and cost-effective method is needed for high-throughput validation of candidate CNV associations and for subsequent routine deployment in diagnostic settings. The predominant method used to validate CNVs in larger populations is real-time or...

The performance of antigen galactomannan (GM) for diagnosing invasive aspergillosis (IA) is hampered by the occurrence of false-positive results. Quantitative PCR has been proposed to improve the diagnosis of IA. Therefore, we analyzed the value of performing a PCR test to the GM-positive serum sample. Using a quantitative PCR assay specific for Aspergillus fumigatus 28S ribosomal DNA, we retro...

The bacterium, Acidovorax avenae ssp. avenae causes several important plant diseases including bacterial stripe of rice, bacterial stalk rot of corn, bacterial leaf blight of oats, and red stripe of sugarcane and millet. Although the organism is seed-borne in rice, no reliable seed assay is available. A semiselective liquid medium based on d-sorbitol and l-pyroglutamic acid (SP medium), and two...

Objective: We aimed to analyse the positivity rate and cycle threshold values indicating viral loads for SARS CoV-2 among different respiratory specimens. Additionally, we evaluated diagnostic efficacy of saliva samples. Patients Methods: included combined oropharyngeal nasopharyngeal swab (cONS), sputum, tracheal aspirate (TA) specimens patients. Unpreserved samples were collected prospectivel...

three hundred twin births from a hospital in tehran, iran have been analyzed for seasonal variation at birth and physical measurements. the results show that the highest twinning rates for mm (5.61), ff (7.73) and mf (5.36) were seen in april, january and september, respectively. the four physical measurements at birth showed non-significant differences among the male and female twins, but mm a...

a experiment was lay out in order to evaluate the effects  of different biofertilizers on yield and yield components of maize at the lorestan provience, iran. the experiment was a factorial design with three replications. treatments were three nitrogen biofrtilizers (nitrokara (n1), o4 (n2), o6 (n3) and control (n4)) and three phosphate biofrtilizers (phosphate barvar2 (p1), biozarr (p2), mc1+p...

studies show that any complications including hemorrhage, lack of blood supply, lack of oxygen supply, and death of cells in a tissue, will have a clear effect on electrical properties of that tissue. thus, by measuring impedance of a set of tissues, potential problems of the damaged tissue may be found. since electrical impedance is closely related to the measuring frequency, obviously, every ...

introduction: birth weight is one of the important sanitary indices in evaluating the prenatal care all over the world. the purpose of this research was to determine the relationship between the bio-environmental characteristics of mothers with infant’s birth weight. materials and methods: in corrolation study, first, all newborn babies in hospitals and maternity centers khuzestan between farwa...

The Centers for Disease Control and Prevention and United States Army Research Institute for Infectious Diseases have developed real-time PCR assays for the detection of bioterrorism threat agents. These assays all rely on a limited number of approved real-time PCR master mixes. Because the availability of these reagents is a critical element of bioterrorism preparedness, we undertook a joint n...