According to the Gα subunit amino acid sequence homology, G proteins divide into four families that can couple to different GPCRs. To investigate the combination of interaction between G proteins (Gs, Gi/Go, q11 and 12/13) and A2A, D2R, or A2A/D2 respectively, Föster resonance energy transfer (FRET) and bimolecular fluorescence complementation (BiFC) assays, with the advantage to analyze protei...

According to the Gα subunit amino acid sequence homology, G proteins divide into four families that can couple to different GPCRs. To investigate the combination of interaction between G proteins (Gs, Gi/Go, q11 and 12/13) and A2A, D2R, or A2A/D2 respectively, Föster resonance energy transfer (FRET) and bimolecular fluorescence complementation (BiFC) assays, with the advantage to analyze protei...

H-Ras is a binary switch that is activated by multiple co-factors and triggers several key cellular pathways one of which is MAPK. The specificity and magnitude of downstream activation is achieved by the spatio-temporal organization of the active H-Ras in the plasma membrane. Upon activation, the GTP bound H-Ras binds to Galectin-1 (Gal-1) and becomes transiently immobilized in short-lived nan...

Targeting protein-protein interactions (PPI) is an emerging field in drug discovery. Dimerization and PPI are essential properties of human immunodeficiency virus (HIV)-1 proteins, their mediated functions, and virus biology. Additionally, dimerization is required for the functional interaction of HIV-1 proteins with many host cellular components. In this study, a bimolecular fluorescence compl...

A defining characteristic of HIV-1 infection is the ability of the virus to persist within the host. Specifically, MHC-I downregulation by the HIV-1 accessory protein Nef is of critical importance in preventing infected cells from cytotoxic T-cell mediated killing. Nef downregulates MHC-I by modulating the host membrane trafficking machinery, resulting in the endocytosis and eventual sequestrat...

Occludin (Ocln), a MARVEL-motif-containing protein, is found in all tight junctions. MARVEL motifs are comprised of four transmembrane helices associated with the localization to or formation of diverse membrane subdomains by interacting with the proximal lipid environment. The functions of the Ocln MARVEL motif are unknown. Bioinformatics sequence- and structure-based analyses demonstrated tha...

Although multiple lines of evidence have indicated that Arabidopsis thaliana Tandem CCCH Zinc Finger proteins, AtTZF4, 5 and 6 are involved in ABA, GA and phytochrome mediated seed germination responses, the interacting proteins involved in these processes are unknown. Using yeast two-hybrid screens, we have identified 35 putative AtTZF5 interacting protein partners. Among them, Mediator of ABA...