In a two-compartment scintillation vial, suspensions of bacteria were cultured with 1 muCi of [U-14C] glucose and the released 14C02 was measured continuously, cumulatively, and automatically in a liquid-scintillation counter modified to maintain sample temperature at 37 degrees C. We could follow the metabolism of bacterial populations through their early phase of exponential growth with good ...

Bacterial colony enumeration is an essential tool for many widely used biomedical assays. This chapter introduces a cost-effective and fully automatic bacterial colony counter which accepts digital images as its input. The proposed counter can handle variously shaped dishes/plates, recognize chromatic and achromatic images, and process both color and clear medium. In particular, the counter can...

This thesis covers a myriad of interconnected topics concerning biological micro-organisms and synthetic micro-robots. Via intricate propulsion strategies these micro-swimmers can move through strongly viscosity-dominated liquids to achieve their goals, be it to invade new territories or to deliver drugs to infected regions. Therefore, the understanding of bacterial motility immediately impacts...

Enterotoxigenic Escherichia coli K99 is one of the dominant pathogens associated with diarrhea of calves. Immunoglobulin Y (IgY), has been used as an inexpensive alternative to antibiotics for the prevention and therapy of several bacterial infections. The study aimed to prepare IgY antibodies against E. coli K99 and to investigate its in vitro effectiveness. E. c...

The concentration of estrogen receptor protein in breast-tumortissue is generallyexpressed in units of femtomoles of estradiol bound by the receptor per milligram of cytosol protein. The sensitivity of the estrogen receptor radioligand assay is therefore related to the specific activity of the steroid label used for the binding assay, the amount of the receptor protein in the volume of cytosolu...

We evaluated a broad-based PCR assay coupled with high-resolution melt analysis for rapid bacterial identification in patients with bacterial sepsis. With a reference library of 60 clinically relevant bacterial species, 52 positive blood culture samples were tested. Our assay identified 46/52 samples at the species level, with 100% concordance to culture findings.

Brilliant blue is largely found in ice cream, canned processed peas, packet soups, blue raspberry flavored products, dairy products, sweets and drinks. It is also used in soaps, shampoos, mouthwash and other hygiene and cosmetics applications (Archived from Florida). This study was aimed to investigate the antimicrobial effects of Brilliant blue on Bacillus subtilis as a gram positive bacterial...