We developed single-point genome signature tags (SP-GSTs), a generally applicable, high-throughput sequencing-based method that targets specific genes to generate identifier tags from well-defined points in a genome. The technique yields identifier tags that can distinguish between closely related bacterial strains and allow for the identification of microbial community members. SP-GSTs are det...

Screening of3000 soil samples collected from cities of four different provinces of Iran for starch-hydrolysing bacteria revealed that the nature is enriched with Streptomyces species capable of producing amylolytic enzymes. Among the bacterial isolates, one of the high starch-degrading strains was selected for further microbiological identification and also amylolytic enzyme(s) characteriza...

Microbial degradation of lignin, a natural complex biopolymer, renewable raw material with wide range applications, has been mainly directed at fungal systems, nevertheless, recent studies have proposed the bacterial role in lignin and modification since bacteria possess remarkable environmental adaptability, various production enzymes biochemistry. An occurrence high proportion lignin-degradin...

OXA-48-type ?-lactamases are now routinely encountered in bacterial infections caused by carbapenem-resistant Enterobacterales . These enzymes of high and growing clinical significance due to the importance carbapenems treatment health care-associated Gram-negative bacteria, wide increasing dissemination OXA-48 on plasmids, challenges posed their detection.

Artificial designer minicellulosomes comprise a chimeric scaffoldin that displays an optional cellulose-binding module (CBM) and bacterial cohesins from divergent species which bind strongly to enzymes engineered to bear complementary dockerins. Incorporation of cellulosomal cellulases from Clostridium cellulolyticum into minicellulosomes leads to artificial complexes with enhanced activity on ...

Bacterial cellulose is a unique biopolymer that has found numerous biomedical applications, such as being an excellent wound-dressing material or carrier for delivering active compounds. The purpose of this study was to analyze the ability modified bacterial (BC) using low-pressure Ar plasma control release glycoside hydrolases with antibiofilm activity, namely PelAh and PslGh, from Pseudomonas...

OBJECTIVE The activity of lysosomal enzymes is increased in the cerebrospinal fluid of bacterial meningitis, suggesting that inflammation may cause leakage of lysosomal enzymes into the extracellular fluid. Our objective was to study the activity of 3 lysosomal enzymes in cell-free peritoneal fluid of patients with peritoneal inflammation. METHODS The beta-galactosidase, beta-glucuronidase, a...

The widespread use of carbon nanotubes (CNTs) has raised concerns about the human health and ecological effects CNTs released into environment. Bacteria play an important role in bioremediation waste treatment, their enzymes are mostly responsible for degradation contaminants. However, there still only a few reports bacterial CNTs, evidence showing involvement CNT with mechanisms never been rep...

Cellulose agricultural waste is a source of environmental pollution and an alternative solution to overcome it degrade this with the help cellulolytic enzymes. The Biochemistry Laboratory FMIPA Riau University has number endophytic bacterial isolates from roots Mangrove Ceriops tagal (Perr) C. B. Rob in Bengkalis area whose potential produce enzymes not yet known. Qualitative selection carboxy ...