Conjugational transfer of pLS20 in Bacillus subtilis Marburg 168 is restricted by the BsuM restriction-modification system. Restriction efficiency was measured using pLS20 derivatives possessing various numbers of XhoI sites, which are known to be recognized by BsuM. An increase in XhoI sites clearly reduced the conjugational efficiency of pLS20 as compared with that of pUB110 plasmid lacking X...

Bacillus represents microbes of high economic, medical and biodefense importance. Bacillus strain identification based on 16S rRNA sequence analyses is invariably limited to species level. Secondly, certain discrepancies exist in the segregation of Bacillus subtilis strains. In the RDP/NCBI databases, out of a total of 2611 individual 16S rDNA sequences belonging to the 175 different species of...

background and aims: the ectodomain of matrix protein of influenza virus is a weak immunogen that is highly conserved among all subtypes of influenza a virus. tandem repeats of these genes along with linker were used to enhance immunogenicity of m2e protein and so it can be served as a universal vaccine in both humans and livestock. materials and methods: in this study, the sequences of extra-d...

in this study, 5,10,15,20-tetrakis(4-nitrophenyl)porphyrin (tnpp) and its zinc porphyrin complex (zntnpp) were synthesized in situ using the microwave method and identified by uv-vis, ft-ir and 1h nmr. the photostability and photodynamic antimicrobial activity (pact) of these compounds were investigated on pseudomonas aeruginosa and bacillus subtilis under visible light irradiation. mic, mbc an...

the present study was undertaken to screen the soil samples collected in iran for the presence of the bacillus subtilis lipase a gene. the bacterial colonies obtained from the collected soil samples were examined by physical appearance, biochemical tests and  the polymerase chain reaction (pcr). only four colonies were identified as putative b. subtilis strains and all contained the lipase a ge...