Bacteria and actinomycetes were screened for esterase enzymes capable of removing the para-nitrobenzyl ester from cephalosporins. An esterase preparation from Bacillus subtilis was used to prepare cephalexin and 7-ADCA from the corresponding para-nitrobenzyl esters.

We describe a method for obtaining synchronously dividing cells of bacteria (Escherichia coli B and K-12 and Bacillus subtilis 168) and fission yeasts (Schizosaccharomyces pombe) by the use of Percoll density gradients.

antimicrobial activity of the roots of eranthemum roseum (vahl) r.br. (dasmuli), were tested against different bacteria (including escherichia coli, staphylococcus aureus, bacillus subtilis, shigella sonnei, klebsiella pneumoniae, salmonella typhi, proteus vulgaris, pseudomonas aeruginosa) and fungi (such as candida albicans and aspergillus niger) by cup plate diffusion method. minimal inhibito...

During the course of an experimental study of bacteriuria, specimens of urine thought to contain Bacillus prodigiosus which had been injected into the circulation became contaminated with Bacillus subtilis. In an effort to rid them of this contamination, the effect on the two organisms of a number of common dyes was studied and a striking selective power possessed by gentian violet was brought ...

Background: Antibacterial proteins are widely expressed in snake venoms. Previously, we have isolated two immunodominant proteins with molecular weights of 14 and 65kD from the snake venom of Naja naja oxiana (N. oxiana). It was demonstrated that they had potent inhibitory effects against gram-positive bacteria, S. aureus and B. subtilis but were less effective against gram-negative bacteria, s...

AmyL, an extracellular alpha-amylase from Bacillus licheniformis, is resistant to extracellular proteases secreted by Bacillus subtilis during growth. Nevertheless, when AmyL is produced and secreted by B. subtilis, it is subject to considerable cell-associated proteolysis. Cell-wall-bound proteins CWBP52 and CWBP23 are the processed products of the B. subtilis wprA gene. Although no activity h...

The effect of viscosity on the swimming speed and motion pattern four kinds microswimmers different sizes which move by contrasting propulsion mechanisms was analyzed: two biological swimmers (bovine sperm cells Bacillus subtilis bacteria) bending patterns their flagella artificial with catalytic (alginate microtubes Janus Pt@SiO2 spherical microparticles).

The major metabolites of Diopsyros melanoxylon viz. amyrins and ursolic acid and their lipophilic 3-O-fatty acid ester chains (C12-C18), which are synthesized now under mild esterification conditions in excellent yields (80-95%), were evaluated for their antimicrobial activity against a series of Gram positive and Gram negative bacteria. Significantly these compounds were found to exhibit poten...

The ftsL gene is required for the initiation of cell division in a broad range of bacteria. Bacillus subtilis ftsL encodes a 13-kDa protein with a membrane-spanning domain near its N terminus. The external C-terminal domain has features of an alpha-helical leucine zipper, which is likely to be involved in the heterodimerization with another division protein, DivIC. To determine what residues ar...