A total of 3 strains were found with potential antibacterial activity. The MMKK5 strain showed moderate, good and excellent growth in GAA broth during 7, 14 and 21 day of incubation respectively while in starch-casein broth weak growth observed in 7 days incubation but finally at twenty one days showed excellent growth. MMKK2 showed moderate to good growth up to 21 days of incubation. Whereas, ...

Bacillus spore surface morphology was imaged with atomic force microscopy (AFM) to determine if characteristic surface features could be used to distinguish between four closely related species; Bacillus anthracis Sterne strain, Bacillus thuringiensis var. kurstaki, Bacillus cereus strain 569, and Bacillus globigii var. niger. AFM surface height images showed an irregular topography across the ...

The present study includes the collection, isolation and characterization of microorganisms from the cow dung of local varieties from different places of Dehradun, Uttrakhand, India. Four different strains of bacteria B1, B3, B4, B5 were isolated in which three are gram positive (cocci form) and one is gram-negative (bacillus form). Only one strain shows (B4) the formation of endospore. The enz...

Here we present vB_BanS-Tsamsa, a novel temperate phage isolated from Bacillus anthracis, the agent responsible for anthrax infections in wildlife, livestock and humans. Tsamsa phage is a giant siphovirus (order Caudovirales), featuring a long, flexible and non-contractile tail of 440 nm (not including baseplate structure) and an isometric head of 82 nm in diameter. We induced Tsamsa phage in s...

Several products of industrial interest are produced by Bacillus, including enzymes, antibiotics, amino acids, insecticides, biosurfactants and bacteriocins. This study aimed to investigate the potential of two bacterial isolates (P5 and C3) from puba, a regional fermentation product from cassava, to produce multiple substances with antimicrobial and surface active properties. Phylogenetic anal...

Plant growth promoting (PGP) rhizobacteria, a beneficial microbe colonizing plant roots, enhanced crop productivity and offers an attractive way to replace chemical fertilizers, pesticides, and supplements. The keratinous waste which comprises feathers, hairs, nails, skin and wool creates problem of solid waste management due to presence of highly recalcitrant keratin. The multi traits rhizobac...

The architecture of spore surfaces plays an important role in the pathogenesis and natural ecology of many Bacillus organisms. However, changes to the laboratory preparation method for Bacillus spores may be a source of phenotypic variability in cell surfaces that can affect our understanding of in situ morphology and make cross-study comparisons more difficult. In this work, we examined how va...

Bacillus thuringiensis represents one of the six species of "Bacillus cereus group" in the genus Bacillus within the family Bacillaceae. Strain Sbt003 was isolated from soil and identified as B. thuringiensis. It harbors at least seven plasmids and produces three shapes of parasporal crystals including oval, bipyramidal and rice. SDS-PAGE analysis of spore-crystal suspension of this strain reve...

The identification of Bacillus anthracis involves its differentiation from other aerobic sporeformers, particularly Bacillus cereus. Smith et al. (1952), in a study of aerobic sporeforming bacteria, concluded that B. anthracis is a pathogenic variety of B. cereus. They also stated that strains of B. anthracis which had lost their virulence could not be differentiated from B. cereus. This appare...