pim1-d1ts was previously identified in a visual screen for fission yeast mutants unable to complete the mitosis-to-interphase transition. pim1+ encodes the guanine nucleotide exchange factor (GEF) for the spi1 GTPase. Perturbations of this GTPase system by either mutation or overproduction of its regulatory proteins cause cells to arrest with postmitotic condensed chromosomes, an unreplicated g...

Vascular endothelial growth factor (VEGF) and its receptors have been implicated in promoting solid tumor growth and metastasis via stimulating tumor-associated angiogenesis. Models of murine tumor angiogenesis and receptor-specific antibodies are required to evaluate roles of VEGF receptors in mouse models of human cancer. Human VEGFR2 (also known as KDR) and murine VEGFR2 (or Flk-1) share 85%...

BACKGROUND Vaccination could induce immune tolerance and protected NOD mice from the development of type I diabetes (T1D). We previously demonstrated that insulin peptide (B9-23) combined with dexamethasone (DEX) stimulated the expansion of antigen specific regulatory T (Treg) cells which in turn effectively prevented T1D in NOD mice. Here, we aimed to investigate the therapeutic effect of tole...

Previous analyses by fluorescence in situ hybridization of structures present 20-30 cell generations after the primary events of mammalian gene amplification have shown that tens of megabases of DNA separate each copy of the selected gene in chromosomal arrays that contain up to 15 copies. Since these structures are very unstable, it is necessary to study amplified DNA as soon as possible after...

This work focused on determining the micronutrient status of Crocus sativus L.stigmas with studying different correlations between physical-chemical compounds (moisture, minerals, ash, total phenolic content, carotenoids, zeaxanthin, vitamins and antioxidant activity). The mineral composition (Na, K, Ca, Mg, P, Fe, Cu, Mn, Zn, Mo B) was analyzed by ICP-OES (Inductively Coupled Plasma - Optical ...

Hydrogels, due to their unique potentials such as high-water content and hydrophilicity are interest for the controlled release of drug molecules. The present study aims to create a controlled-release system through the preparation and characterization of hydrogels based on pH-sensitive polymers such as poly (acrylic acid). Poly (acrylic acid), p(AA), hydrogel has been synthesized by radical po...

WIF-B9 is a suitable model for in vitro studies of hepatocyte polarity. To better understand polarity establishment, we have localized key proteins of the adhesion system, cytoskeleton, and tight junctions soon after plating, when most cells are isolated or in doublets. In isolated attached cells, only cytoskeletal proteins (tubulin, cytokeratins) displayed a precise localization. As soon as tw...

The 3 pair of B9 stars, HD 28867, is one of the brightest X-ray sources in the Taurus-Auriga star forming region. In this multi-wavelength study, we attempt to deduce the source of the X-ray emission. We show that the East component is the X-ray source. The East component has a near-IR excess and displays narrow absorption lines in the optical, both of which are consistent with a cool stellar c...

This work aims to evaluate the potential use of insulin-like growth factor 2 (IGF-2) as the dominant thymic self-antigen precursor of the insulin family in designing a tolerogenic approach to type 1 diabetes (T1D) prevention. This evaluation was primarily based on cytokine profile driven by MHC presentation of insulin and IGF-2-derived antigens to PBMC cultures derived from 16 T1D DQ8(+) adoles...

Cysteine peptidases (CPs) of Entamoeba histolytica are considered to be important pathogenicity factors. Previous studies have found that under standard axenic culture conditions, only four (ehcp-a1, ehcp-a2, ehcp-a5, and ehcp-a7) out of 35 papain-like ehcp genes present in the E. histolytica genome are expressed at high levels. Little is known about the expression of CPs in E. histolytica duri...