Vascular endothelial growth factor A (VEGFA) is a specific mitogen for vascular endothelial cells that plays a critical role in cancer neoangiogenesis. Here, we report that the nucleolar tumor suppressor p19(ARF) suppresses VEGFA expression, acting at the level of mRNA translation without affecting the transcription of the VEGFA gene. Translational repression of VEGFA mRNA by p19(ARF) does not ...

Kanamycin belongs to the aminoglycoside class of bacteriostatic antibiotics that inhibits protein synthesis. Previous studies have shown that treatment with sublethal levels of kanamycin causes the extracellular polysaccharide capsule in E. coli B23 to increase in size, and potentially confers the bacterium with kanamycin resistance. This study attempted to confirm this by establishing that inc...

Dynamic phosphorylation and dephosphorylation of the hepadnavirus core protein C-terminal domain (CTD) are required for multiple steps of the viral life cycle. It remains unknown how the CTD phosphorylation state may modulate core protein functions but phosphorylation state-dependent viral or host interactions may play a role. In an attempt to identify host factors that may interact differentia...

The human pt20 nucleolar protein is a cell cycle-related protein that peaks during the S phase and has been shown to be associated with a beaded fibrillar structure. To study domains responsible for the nucleolar localization of protein p120, initially deletion mutants were made that defined sequences containing the localization signals; then, fusion genes that were composed of segments of the ...

Previous studies have shown that pre-treatment of Escherichia coli B23 with subinhibitory concentrations of aminoglycosides leads to a subsequent increase in the level of capsular polysaccharide, as well as an increased resistance to streptomycin. Using capsular polysaccharide synthesis pathway mutants, we investigated the correlation between the level of capsular polysaccharide and streptomyci...