نتایج جستجو برای: b ketoacyl coa synthase

تعداد نتایج: 993892  

Journal: :Bioscience, biotechnology, and biochemistry 2003
Hirosuke Oku Naoya Futamori Kenichi Masuda Yumiko Shimabukuro Tomoyo Omine Hironori Iwasaki

It was found that the partially purified beta-ketoacyl-ACP synthase of Bacillus insolitus did not require the addition of FabD (malonyl-CoA:ACP transacylase, MAT) for the activity assay. This study therefore examined the necessity of FabD protein for in vitro branched-chain fatty acid (BCFA) biosynthesis by crude fatty acid synthetases (FAS) of Bacilli. To discover the involvement of FabD in th...

2014
Ralph Ewald Christiane Hoffmann Alexandra Florian Ekkehard Neuhaus Alisdair R. Fernie

Prosthetic lipoyl groups are required for the function of several essential multienzyme complexes, such as pyruvate dehydrogenase (PDH), a-ketoglutarate dehydrogenase (KGDH), and the glycine cleavage system (glycine decarboxylase [GDC]). How these proteins are lipoylated has been extensively studied in prokaryotes and yeast (Saccharomyces cerevisiae), but little is known for plants. We earlier ...

Journal: :Biochemistry 2017
Grace A R Thiele Connie P Friedman Kathleen J S Tsai Joris Beld Casey H Londergan Louise K Charkoudian

Acyl carrier proteins (ACPs) are central hubs in polyketide and fatty acid biosynthetic pathways, but the fast motions of the ACP's phosphopantetheine (Ppant) arm make its conformational dynamics difficult to capture using traditional spectroscopic approaches. Here we report that converting the terminal thiol of Escherichia coli ACP's Ppant arm into a thiocyanate activates this site to form a s...

Journal: :Genetics 2008
Michael J Hynes Sandra L Murray Gillian S Khew Meryl A Davis

Peroxisomes are organelles containing a diverse array of enzymes. In fungi they are important for carbon source utilization, pathogenesis, development, and secondary metabolism. We have studied Aspergillus nidulans peroxin (pex) mutants isolated by virtue of their inability to grow on butyrate or by the inactivation of specific pex genes. While all pex mutants are able to form colonies, those u...

پایان نامه :وزارت علوم، تحقیقات و فناوری - دانشگاه شهید چمران اهواز - دانشکده علوم 1394

(mitochondrial trifunctional protein (mtp که در غشای درونی میتوکندی متصل است،سه مرحله نهایی چرخه بتا اکسیداسیون اسید های چرب را کاتالیز میکند.این کمپلکس یک هترو-اکتامر کمپلکس است که از 8 قسمت(زیرواحد) تشکیل شده است. 4زیرواحد ? شامل (lceh(long-chain2,3-enoyl-coahydratase و (lchad (long-chain 3- hydroxyacyl coa dehydrogenase می باشد و 4 زیرواحد ? که در برگیرنده فعالیت (lckt(long chain 3-ketoacyl...

Journal: :Plant physiology 2000
E B Cahoon E F Marillia K L Stecca S E Hall D C Taylor A J Kinney

The seed oil of meadowfoam (Limnanthes alba) and other Limnanthes spp. is enriched in the unusual fatty acid Delta(5)-eicosenoic acid (20:1Delta(5)). This fatty acid has physical and chemical properties that make the seed oil of these plants useful for a number of industrial applications. An expressed sequence tag approach was used to identify cDNAs for enzymes involved in the biosynthesis of 2...

Journal: :Journal of bacteriology 1996
Z Shen D M Byers

We report the isolation of Vibrio harveyi acyl carrier protein (ACP) and cloning of a 3,973-bp region containing the fabG (encoding 3-ketoacyl-ACP reductase, 25.5 kDa), acpP (encoding ACP, 8.7 kDa), fabF (encoding 3-ketoacyl-ACP synthase II, 43.1 kDa), and pabC (encoding aminodeoxychorismate lyase, 29.9 kDa) genes. Predicted amino acid sequences were, respectively, 78, 86, 76, and 35% identical...

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