Atrophy of the gastric mucosa is a precursor of intestinal-type gastric cancer, and Helicobacter pylori infection causes atrophic gastritis. The aim of this study was to determine whether the genetic diversity of H. pylori virulence genes is associated with the development and progression of gastric atrophy in humans. We isolated and cultured H. pylori strains from patients with gastric ulcer a...

Because the mechanisms of Helicobacter pylori-induced gastric injury are incompletely understood, we examined the hypothesis that H. pylori induces matrix metalloproteinase-1 (MMP-1) secretion, with potential to disrupt gastric stroma. We further tested the role of CagA, an H. pylori virulence factor, in MMP-1 secretion. Co-incubation of AGS cells with Tx30a, an H. pylori strain lacking the cag...

The genotypes of Helicobacter pylori flaA, cagA, vacA, and iceA were determined for DNA isolated from patients with chronic gastritis or peptic ulcer in eastern Taiwan. The vacA gene encoding the s1a subtype was found to predominate in peptic ulcer patients, and the iceA1 genotype was associated with chronic gastritis. cagA and flaA genes were not found to be associated with these types of dise...

Pro-inflammatory Interleukin-1 receptor antagonist (IL-1RN) gene polymorphism and high risk Helicobacter pylori virulence factors in combination plays an important role in the outcome of H. pylori associated diseases, which can even lead to gastric cancer. The purpose of this study was to analyze the prevalence of high risk host and bacterial factors such as IL-1RN gene variable number tandem r...

INTRODUCTION Helicobacter pylori strains expressing cytotoxic CagA protein are more commonly associated with peptic ulceration, atrophic gastritis and gastric adenocarcinoma than those lacking CagA. Determination of anti-CagA antibodies, therefore, acquires a relevant clinical significance in the serological detection of H. pylori infection and disease risk prediction. However, the CagA-serolog...

conclusions the most predominant vaca genotypes in our areas were s1/m2 and s1/m1, which regard as the genotypes with more virulence intensity. the h. pylori vacas1a, caga genotypes have a significant relationship with the presence of pud and gc in iranian patients with dyspepsia. results in this study, vaca genotypes s1/m2, s1/m1, s2/m2 and s2/m1 were determined in 43.4%, 19.3%, 13.2% and 6% o...

Chronic infection with Helicobacter pylori, specifically cagA-positive strains, is associated gastric cancer. Thus, measures to prevent H. pylori are required. This study was conducted clarify the prevalence of in community identify source and comprehensively assess risk infection. We collected 90 human faecal samples 73 environmental (water, vegetable, animal samples) from residents an area a ...

A PCR assay for the detection of Helicobacter pylori in gastric biopsy specimens with specific primers for ureC gene amplification (herein referred to as ureC PCR) was compared with other routine invasive methods (culture, the rapid-urease test, and Giemsa staining of histological sections) with samples from a group of 104 consecutive dyspeptic patients. Bacteria were found in 40 (38.5%), 38 (3...

Genotypic variation of Helicobacter pylori is speculated to associate with different clinical outcomes. In Western countries, the gene encoding blood group antigen-binding adhesin (BabA), babA2, is of high clinical relevance and is a useful marker to identify patients who are at higher risk for peptic ulceration and gastric adenocarcinoma, as are vacA and cagA. We investigated the presence of b...