BACKGROUND various MTT assay methods are proposed to obtain the cell survival parameters. OBJECTIVE determining the survival curve characteristics of two cancerous cells of interest based on a common and a novel MTT assay method after exposing them to ionizing radiation. METHODS a common and a novel MTT assay method were used and compared for obtaining the F10B16 melanoma and 4T1 breast ade...

Background: Evaluation of cell viability is momentous in pharmacologic and oncological research. Cell viability evaluation determines cell sensitivity and consequently treatment outcome. Various methods are available to determine cell survival. Each of these methods evaluates different endpoints. Accordingly, determining the correlation between these methods is important. In this study, in orde...

1. Abstract 2. Introduction 3. Materials and methods 3.1. Main reagents and cell culture 3.2. MTT assay 3.3. Comet assay 3.4. Cell cycle analysis by flow cytometry 3.5. The Caspase-3 colorimetric activity assay 3.6. Statistical analysis 4. Results 4.1. MTT assay 4.2. Comet assay 4.3. FCM assay the distribution of cell cycle and cell apoptosis 4.4. The expression of Caspase-3 5. Discussion 6. Ac...

background: various mtt assay methods are proposed to obtain the cell sur- vival parameters. objective: determining the survival curve characteristics of two cancerous cells of interest based on a common and a novel mtt assay method after exposing them to ionizing radiation. method: a common and a novel mtt assay method were used and compared for obtaining the f10b16 melanoma and 4t1 breast ade...

Titanium (IV) and vanadium (V) complexes are highly potent anticancer agents. A challenge in their synthesis refers to their hydrolytic instability; therefore their preparation should be conducted under an inert atmosphere. Evaluation of the anticancer activity of these complexes can be achieved by the MTT assay. The MTT assay is a colorimetric viability assay based on enzymatic reduction of th...

A major problem associated with the chemosensitivity testing of fresh human tumour cells using the MTT assay is the contamination of nonmalignant cells in the tumour tissues. Highly purified fresh human gastric cancer cells could be obtained from 43 solid tumours and eight malignant ascites for the MTT assay. The success rate of the MTT assay was 87.9% (51 of the 58 cases), and the purity of tu...

The MTT reduction assay depends on the ability of metabolically active cells to reduce the tetrazolium salt (3[4,5-dimethylthiazol-2-y1]-2,5-diphenyltetrazolium bromide) to formazan. This study was conducted to examine and validate a simple and less costly MTT test to determine bovine sperm viability and compare the efficiency of this test with a flow cytometer. Fresh ejaculates from eight bull...