An optode was designed for an indirect determination of ascorbic acid by using neocuproine, which has been coated on transparent triacetylcellulose film as a membrane. The proposed method is based on the oxidation of ascorbic acid to dehydroascorbic acid with the Cu(II) and neocuproine reagent. The increase in the absorbance value of the optode at the maximum wavelength of 455 nm was related ...

Previous studies showed that the intact carbon chain of glucose can serve as a precursor for the synthesis of ascorbic acid in the rat (l-3). Evidence for such a precursor relationship came from experiments in which glucose tracers labeled in carbon 1 and carbon 6 were administered to rats, and the L-ascorbic acid isolated from urine was labeled predominantly in carbon 6 and carbon 1, respectiv...

An attempt was made to unravel further the mechanism by which high dietary concentrations of ascorbic acid influence copper metabolism. The addition of ascorbic acid to the diet of rats caused about a twofold increase in plasma ascorbate concentrations and reduced group mean plasma and tissue concentrations of Cu. The effect of 10 g ascorbic acid/kg diet was greater than that of 1 g/kg. Ascorbi...

An attempt was made to unravel further the mechanism by which high dietary concentrations of ascorbic acid influence copper metabolism. The addition of ascorbic acid to the diet of rats caused about a twofold increase in plasma ascorbate concentrations and reduced group mean plasma and tissue concentrations of Cu. The effect of 10 g ascorbic acid/kg diet was greater than that of 1 g/kg. Ascorbi...

Ascorbic acid is an effective antioxidant and free radical scavenger. Therefore, it is expected that ascorbic acid should act as a radioprotectant. We investigated the effects of post-radiation treatment with ascorbic acid on mouse survival. Mice received whole body irradiation (WBI) followed by intraperitoneal administration of ascorbic acid. Administration of 3 g/kg of ascorbic acid immediate...

Previous model studies have suggested ascorbic acid as one of the major sources of furan, a possibly hazardous compound found in thermally processed foods (e.g. canned products, jars). The study showed that about 2 mmol mol(-1) furan was obtained when dry-heating ascorbic acid, while much lower amounts were formed upon pressure cooking, i.e. 58 micromol mol(-1) at pH 4 and 3.7 micromol mol(-1) ...

introduction atherosclerosis is a disease which begins during childhood. oxidation of lipoproteins is an important factor implicated in atherosclerosis. vitamin c decreases blood serum lipids and may inhibit atherosclerosis. this study was designed to observe the effect of ascorbic acid on blood serum lipids in male rabbits fed high-cholesterol diet. methods twenty white male rabbits were weigh...

BACKGROUND Both resveratrol and vitamin C (ascorbic acid) are frequently used in complementary and alternative medicine. However, little is known about the underlying mechanisms for potential health benefits of resveratrol and its interactions with ascorbic acid. METHODS The antioxidant enzymes heme oxygenase-1 and paraoxonase-1 were analysed for their mRNA and protein levels in HUH7 liver ce...

Metabolic conversion of l-galactono-1, 4-lactone and l-ascorbic acid to (+)-tartaric acid and oxalic acid has been studied in Pelargonium crispum, cv. Prince Rupert. Experiments with specifically labeled substrates suggest a path of conversion involving cleavage of l-ascorbic acid, or a metabolic product of l-ascorbic acid, between C(2) and C(3), such that oxalic acid arises from the two carbon...

The inhibiting effect of ascorbic acid, citric acid and sodium chloride on Polyphenoloxidase (PPO) of Golden Delicious apple cubes was studied. Dipping in ascorbic acid (0.2-10 g/L range) and in NaCl (0.2-1 g/L range) solutions for 5 min increases the PPO activiry. Citric acid solutions (0.2-10 g / L range) have little or no inhibition of PPO. A 90-100% PPO inhibition was obtained with a 5 min ...