Trypsin catalyses the hydrolysis of suitable derivatives of basic amino acids including peptides, amides and esters. Esters are the most sensitive substrates, and it was decided to use them in the present study, which had as one of its aims the elucidation of the relationship between the length of the side chain and susceptibility to hydrolysis by trypsin. Previous investigations had shown that...

It has been shown by Troll et al. (1) that plasmin (present in the blood in an inactive form) can hydrolyze esters of arginine and lysine. These authors used a form01 titration method to follow the action of plasmin on TAME (p-toluenesulfonyl-L-arginine methyl ester) and several precipitation and titration methods (2) to follow the action of the enzyme on LME (L-lysine methyl ester). They also ...

This experiment is an investigation of used cooking oil as a sustainable and renewable resource for methyl ester production. with more than 95.5% production yield, thermally activated hydrated cement has been carefully discussed as a catalyst in the transesterification of used cooking oil into methyl ester to use waste cement as a heterogeneous catalyst for methyl ester production through elect...

Superoxide (O-2) and nitric oxide (NO) act to kill invading microbes in phagocytes. In macrophages NO is synthesized by inducible nitric oxide synthase (iNOS, NOS 2) from L-arginine (L-Arg) and oxygen; however, O-2 was thought to be produced mainly by NADPH oxidase. Electron paramagnetic resonance (EPR) spin trapping experiments performed in murine macrophages demonstrate a novel pathway of O-2...

Objective(s): This study was performed to investigate the effects of mucosal acidification on mRNA expression and protein synthesis of cystathionine gamma lyase (CSE), cystathionine beta synthase (CBS), and mucosal release of H2S in gastric mucosa in rats. Materials and Methods:Thirty-two rats were randomly assigned into 4 groups (8 in each), including: the control group, HCl (10 mM) treated g...

background: cocaine poisoning is known for causing severe clinical effects such as tachycardia, hypertension, agitation and confusion. absence of clinical manifestations in cocaine poisoning is unusual. case report: a 26-year old man, known to be a cocaine addict, declared that he was forced by cocaine dealers to swallow many tablets of cocaine, six hours prior to admission to emergency departm...