Geobacillus stearothermophilus T-6 is a thermophilic soil bacterium that has a 38-kb gene cluster for the utilization of arabinan, a branched polysaccharide that is part of the plant cell wall. The bacterium encodes a unique three-component regulatory system (araPST) that includes a sugar-binding lipoprotein (AraP), a histidine sensor kinase (AraS), and a response regulator (AraT) and lies adja...

1,2,4-butanetriol (butanetriol) is a useful precursor for the synthesis of the energetic material butanetriol trinitrate and several pharmaceutical compounds. Bacterial synthesis of butanetriol from xylose or arabinose takes place in a pathway that requires four enzymes. To produce butanetriol in plants by expressing bacterial enzymes, we cloned native bacterial or codon optimized synthetic gen...

Bacillus subtilis produces hemicellulases capable of releasing arabinosyl oligomers and arabinose from plant cell walls. In this work, we characterize the transcriptional regulation of three genes encoding arabinan-degrading enzymes that are clustered with genes encoding enzymes that further catabolize arabinose. The abfA gene comprised in the metabolic operon araABDLMNPQ-abfA and the xsa gene ...

Crude cell-free preparations of Pseudomonas saccharophila grown on n-arabinose oxidize n-arabinose to ol-ketoglutaric acid (1). n-Arabonic acid has been identified as an intermediate in this reaction. The investigations described in this communication are concerned with the mechanism of the conversion of Larabonate to a-ketoglutarate. Evidence is presented for the intermediate formation of &2-k...

Dobrogosz, Walter J. (University of Illinois, Urbana) and Ralph D. DeMoss. Induction and repression of l-arabinose isomerase in Pediococcus pentosaceus. J. Bacteriol. 85:1350-1355. 1963.-The inducible l-arabinose isomerase of Pediococcus pentosaceus can be rapidly and conveniently measured in whole-cell preparations by use of a standard colorimetric procedure originally developed for studies wi...

Using a single-RNA detection technique in live Escherichia coli cells, we measure, for each cell, the waiting time for the production of the first RNA under the control of PBAD promoter after induction by arabinose, and subsequent intervals between transcription events. We find that the kinetics of the arabinose intake system affect mean and diversity in RNA numbers, long after induction. We ob...

It has recently been demonstrated (Borzani et al., 1956) that the mash obtained from Araucaria brasiliensis seeds by acid hydrolysis, when fermented by Saccharomyces cerevisiae, give alcohol yields practically constant at pH values from 2.9 to 7.2. This fact represents a considerable increase of the yeast optimum pH interval. It has also been observed (Borzani et al., 1956) that glucose and ara...

Mutations in the arabinose transport operons of Escherichia coli K-12 were isolated with the Mu lac phage by screening for cells in which beta-galactosidase is induced in the presence of L-arabinose. Standard genetic techniques were then used to isolate numerous mutations in either of the two transport systems. Complementation tests revealed only one gene, araE, in the low-affinity arabinose up...

Crude cell-free preparations of Pseudomonas saccharophila grown on n-arabinose oxidize n-arabinose to ol-ketoglutaric acid (1). n-Arabonic acid has been identified as an intermediate in this reaction. The investigations described in this communication are concerned with the mechanism of the conversion of Larabonate to a-ketoglutarate. Evidence is presented for the intermediate formation of &2-k...