was precipitated (Kane et al., 1975) with 1,1,3,3-tetramethylurea, extracted with chloroform/methanol (1 : 1, v/v), washed with ether, and dried under N2. Amino acid analysis of this protein corresponded to that of apolipoprotein B, as did its electrophoretic mobility on urea/polyacrylamide-gel electrophoresis. Its specific radioactivity was obtained by radioactivity counting and protein determ...

objectives: this study was performed to investigate the effect of ω-3 fatty acids on lipid profiles, lipoprotein (a), apolipoprotein a-1 and apolipoprotein b, fasting glucose and insulin, and insulin resistance in a group of iranian elderly subjects. methods & materials: in this clinical trial, 124 elderly residents of the kahrizakcharity foundation, aged 65 and older were divided into two inte...

We have studied the influence of triglyceride-rich particles on the analytical bias of apolipoprotein B measurements by various immunoturbidimetric methods. Three commercially available methods grossly overestimate apolipoprotein B in samples with even moderately above-normal triglyceride concentrations. This effect is due to the increased relative reactivity of very-low-density lipoproteins in...

Familial defective apolipoprotein B-100 is a genetic disorder of apolipoprotein B-100 that causes moderate to severe hypercholesterolemia. A single amino acid mutation in apolipoprotein B diminishes the ability of low density lipoproteins to bind to the low density lipoprotein receptor. Low density lipoproteins accumulate in the plasma because their efficient receptor-mediated catabolism is dis...

Non-enzymatic glycation of low-density lipoprotein (LDL) may contribute to the premature atherogenesis of patients with diabetes mellitus. To assess whether glycation of apolipoprotein B, the predominant protein of LDL, interferes with the ability to immunologically quantify this protein, we prepared and purified glycated LDL by incubating normal plasma samples with high concentrations of gluco...

Background and Aims: Apolipoprotein B (apoB)-containing triglyceride-rich lipoproteins low-density (LDL) are each causal for myocardial infarction atherosclerotic cardiovascular disease; however, the relative importance is unknown. We tested hypothesis that same number of apoB-containing particles from smaller LDL through to larger lipoproteins, risk similar.