Background: Pseudomonas aeruginosa is an opportunistic pathogen and one of the important factors of hospital infection. It causes many issues such as urinary tract infections, respiratory infection in cystic fibrosis patients, and wound infection in burn patients, septicemia and meningitis. Antibiotic resistance through various mechanisms is one of the challenges for the treatment of pseudomona...

Abstract Background Pseudomonas aeruginosa is a common opportunistic pathogenic bacterium with the ability to develop strong communication pathway by quorum sensing system and different virulent factors. Among various important secretions of P. rhamnolipid biological detergent, believed be involved in development biofilm intercellular communication. It readily dissolves lung surfactants that ar...

Three clones of E. coli, one of which was harbouring a tetracycline resistance plasmid were inoculated together into the stomach of axenic mice. Without antibiotic selective pressure, the R-Plasmid bearing strain became dominant in the faeces of mice, while the R-plasmid free strain was eliminated. When the R-plasmid bearing strain was given to mice 4 days after the inoculation with the R-plasm...

Selectable marker genes (SMGs) have been extraordinarily useful in enabling plant transformation because of the low efficiency of transgene integration. The most used SMGs encode proteins resistant to antibiotics or herbicides and use negative selection, i.e., by killing nontransgenic tissue. However, there are perceived risks in wide-scale deployment of SMG-transgenic plants, and therefore res...

Abstract In a previous study, we reported transient transformation system using repeated screening for hygromycin B (Hyg) resistance in the basidiomycete Ceriporiopsis subvermispora . present by combining this technique with CRISPR/Cas9, demonstrated successful marker-free genome editing Pleurotus ostreatus , which is one of most economically important cultivated mushrooms as well model white-r...