Anion-Exchange Chromatography I. Mechanism of Separation Although anion-exchange chromatography has been used extensively to analyze acidic carbohydrates and glycopeptides, it has not been commonly used for analysis of neutral sugars. However, examination of the pKa values of the neutral monosaccharides listed in Table 1 shows that carbohydrates are in fact weak acids. At high pH, they are at l...
