We give several new criteria for a quasi-projective variety to be affine. In particular, we prove that an algebraic manifold Y with dimension n is affine if and only if H(Y,ΩjY ) = 0 for all j ≥ 0, i > 0 and κ(D,X) = n, i.e., there are n algebraically independent nonconstant regular functions on Y , where X is the smooth completion of Y , D is the effective boundary divisor with support X−Y and...