A cDNA clone that encodes the 14-kDa bifunctional inhibitor from corn seeds (L. Wen et al., Plant Mol. Biol. 18, 813-814, 1992) has been expressed in Escherichia coli after being incorporated into the pT7 expression vector. This inhibitor protein, referred to as CHFI (for the corn inhibitor of activated Hageman factor) or as the popcorn inhibitor, is an important tool for specific inhibition of...

Endophytic bacteria are sized microorganisms found in plant tissues that known to produce secondary metabolites, one of which is the ?-amylase inhibitor. Plant potential closely related endophytic present tissues. The study aimed determine rubber plants inhibitors and identify molecular using 16S rRNA gene sequence. isolates used were L1M1.A2, L1M1.B4, L2M1.A27. activity inhibitor was tested by...

Cordycepin, an inhibitor of RNA synthesis in barley (Hordeum vulgare L.) aleurone cells, does not inhibit the gibberellic acid-enhanced alpha-amylase (EC 3.2.1.1.) synthesis in barley aleurone layers if it is added 12 hours or more after the addition of the hormone. However, the accumulation of alpha-amylase activity after 12 hours of gibberellic acid can be decreased by abscisic acid. The accu...

To determine the role of 1,2-diacylglycerol (1,2-DAG) and protein kinase C in pancreatic enzyme secretion, we measured the effect of various pancreatic secretagogues on the cellular mass of 1,2-DAG and amylase release in dispersed pancreatic acini from the guinea pig. In addition, we measured the effect of a recently described protein kinase C inhibitor 1-(5-isoquinolinesulfonyl)-2-methylpipera...