Molecular markers are used in plant science a variety of ways. The method molecular genome marking based on RAPD-PCR makes it possible to determine the genetic status populations and establish intrapopulation relationships. In this work, we studied polymorphism dandelion Taraxacum officionale using RAPD markers. Five random amplified polymorphic DNA (RAPD) were diversity relationship between tw...

The genetic heterogeneity of nitrite reductase gene (nirK and nirS) fragments from denitrifying prokaryotes in forested upland and marsh soil was investigated using molecular methods. nirK gene fragments could be amplified from both soils, whereas nirS gene fragments could be amplified only from the marsh soil. PCR products were cloned and screened by restriction fragment length polymorphism (R...

Introgression lines are some of the most important germplasm for breeding applications and other research conducted on cotton crops. The DNA methylation level among 10 introgression lines of cotton (Gossypium hirsutum) and three exotic parental species (G. arboreum, G. thurberi and G. barbadense) were assessed by methylation-sensitive amplified polymorphism (MSAP) technology. The methylat...

Amplified fragment length polymorphisms are a PCR-based genetic tool used to mark the presence or absence of alleles in a DNA sample. This tool has typically been used for genetic analysis in plants, but research is now being done to see if it is a reliable source for paternity analysis in animals. Samples of Orconectes limosus were collected from Quinebaug River during a previous study and mat...

conclusion the high discriminative power of hsp65 pattern particularly hpaii digestion, provide an exact and costeffective method for rapid identification of m. simiae strains among registered pulmonary cases. results of 962 culture positive specimens, 17(1.7%) were identified as m. simiae species; majority of them were multidrug-resistance (12; 70.5%). the overall detection rate by tb11, tb12 ...

BACKGROUND AND OBJECTIVES DNA ladder contains DNA fragments of different length but with known size, used to determine the size of unknown DNA molecules. Different DNA ladders are available for expected DNA length. Conserved sequences were selected for design of primers to generate DNA fragments of known specific size. MATERIALS AND METHODS In this study, we describe a method by which DNA lad...

Over 100 genes of resistance to rust fungi: Puccinia recondita f. sp. tritici, (47 Lr - leaf rust genes), P. striiformis (18 Yr - yellow rust genes) and P. graminis f. sp. tritici (41 Sr - stripe rust genes) have been identified in wheat (Triticum aestivum L.) and its wild relatives according to recent papers. Sixteen Lr resistance genes have been mapped using restriction fragments length polym...

Genotypic variability among 96 Trichophyton rubrum strains which displayed different colony morphologies and were collected from four continents was investigated. Twelve markers representing 57 loci were analyzed by PCR fingerprinting, amplified fragment length polymorphism, and random amplified monomorphic DNA markers. Interestingly, none of the methods used revealed any DNA polymorphism, indi...

Using a sequenced pectate lyase-encoding gene (pel gene), we developed a PCR test for Erwinia carotovora. A set of primers allowed the amplification of a 434-bp fragment in E. carotovora strains. Among the 89 E. carotovora strains tested, only the Erwinia carotovora subsp. betavasculorum strains were not detected. A restriction fragment length polymorphism (RFLP) study was undertaken on the amp...

Two species from the genus Penicillium, Penicillium expansum and P. griseoroseum (Brasilian isolates) were characterized morphologic and molecularlly. Morphological variability was detected among isolates in regard to colony morphology and to conidia coloration. The molecular characterization was based on the RAPD markers, telomeric fingerprinting and ITS sequencing. A total of 78 RAPD primers ...