The neutrophil enzyme myeloperoxidase uses H2O2 to oxidize chloride, bromide, iodide and thiocyanate to their respective hypohalous acids. Chloride is considered to be the physiological substrate. However, a detailed kinetic study of its substrate preference has not been undertaken. Our aim was to establish whether myeloperoxidase oxidizes thiocyanate in the presence of chloride at physiologica...

l:lle or&n and fate of thiocyanate ion in the body have been of interest for more than a century. It has been generally accepted that the ion does not readily permeate normal cell membranes; t.hc distribution of thiocyanate ion in the body is the basis of a method for determination of extracellular fluid volume (1). Despite its assumed extracellular localization, thiocyanate ion is physiologica...

A kinetic spectrophotometric method for rapid measurement of thiocyanate in serum, urine and sewage water with no need for removed of interfering substances in described. The method is based on the inhibitory effect of thiocyanate on the oxidation of pyrogallol red by bromated and nitrite which is monitored at 467 nm. The variables affecting the rate of the reaction were investigated and th...

We describe a method for rapid and specific measurement of thiocyanate in serum or urine. We separate thiocyanate from interfering compounds by adsorbing it on an anion-exchange resin that has special affinity for thiocyanate, then eluting with sodium perchlorate. The eluted thiocyanate is quantified by a modified König reaction, sodium hypochlorite being used as the chlorinating reagent. Analy...

In adequate iodine intake including and a number of environmental agents can affect thyroid gland functions in man and animals. Thiocyanate and catechin that arise for the consumption of cyanogenic and flavonoids containing foods respectively, affect thyroid hormone synthesis but the data comparing the goitrogenic/ antithyroid potentiality of thiocyanate and catechin when act individually or sy...

An analytical method utilizing chemical ionization gas chromatography-mass spectrometry was developed for the simultaneous determination of cyanide and thiocyanate in plasma. Sample preparation for this analysis required essentially one-step by combining the reaction of cyanide and thiocyanate with pentafluorobenzyl bromide and simultaneous extraction of the product into ethyl acetate facilitat...