Nine Clostridium perfringens strains were isolated from fifteen intestinal content samples collected from commercial broilers which suffered from necrotic enteritis NE. Molecular typing of C. perfringens was performed by PCR using three sets of primers specific for toxin -producing genes of C. perfringens [alpha (900bp), beta (236bp) and epsilon (541bp)]. Six strains of C. perfringens type A an...

Hirschsprung's disease (HD) is a development disorder of the enteric nervous system in which the altered innervation explains the inability of the aganglionic segment to relax. Impairment of cytoskeleton in SMC of aganglionic bowel has been shown. Sarcoglycan subcomplex (SG) may support the development and maintenance of muscle cells. We examined the SG subunit expression in colonic aganglionic...

The hypotheses that PKC epsilon is necessary for: 1) PGF2 alpha to inhibit LH-stimulated progesterone (P4) secretion, and 2) for the expression of key prostaglandin synthesizing/metabolizing enzymes were tested in bovine luteal cells in which PKC epsilon expression had been ablated using a validated siRNA protocol. Steroidogenic cells from Day -6 bovine corpus luteum (CL) were isolated and tran...

Circular muscle of the esophagus (ESO) is normally relaxed and contracts phasically in response to neural stimuli. In contrast, lower esophageal sphincter (LES) circular muscle maintains spontaneous tone and relaxes in response to neural stimuli. We have previously shown that in vitro, spontaneous LES tone and contraction of ESO in response to acetylcholine (ACh) are antagonized by protein kina...

Lutein, a dihydroxy derivative of alpha-carotene (beta,epsilon-carotene), is the most abundant carotenoid in photosynthetic plant tissues where it plays important roles in light-harvesting complex-II structure and function. The synthesis of lutein from lycopene requires at least four distinct enzymatic reactions: beta- and epsilon-ring cyclizations and hydroxylation of each ring at the C-3 posi...

Let $$X_1,\ldots ,X_n$$ be an i.i.d. sample from symmetric stable distribution with stability parameter $$\alpha$$ and scale $$\gamma$$ . $$\varphi _n$$ the empirical characteristic function. We prove a uniform large deviation inequality: given preciseness $$\epsilon >0$$ probability $$p\in (0,1)$$ , there exists universal (depending on $$\epsilon$$ p but not depending ) constant $$\bar{r}>0$$ ...

The T cell receptor for antigen (TCR) is a multisubunit complex that consists of at least seven polypeptides: the clonotypic, disulfide-linked alpha/beta heterodimer that is noncovalently associated with the invariant polypeptides of the CD3 complex (CD3-gamma, -delta, -epsilon) and zeta, a disulfide-linked homodimer. We achieved the complete assembly of the human TCR in an in vitro transcripti...

We describe a quantum algorithm to estimate the $\ensuremath{\alpha}$-Renyi entropy of an unknown density matrix $\ensuremath{\rho}\ensuremath{\in}{\mathbb{C}}^{d\ifmmode\times\else\texttimes\fi{}d}$ for $\ensuremath{\alpha}\ensuremath{\ne}1$ by combining recent technique singular value transformations with method estimating normalized traces in one clean qubit model. consider oracular input mo...

The effect of myocardial hypertrophy on passive stiffness was determined from the stress (sigma)-strain relationship of right ventricular papillary muscles from 18 pulmonary artery-banded and 21 non-banded cats. By use of Lagrangian strain, (1-lo)lo is initial muscle length, and instantaneous stress elastic constants beta and alpha were calculated from the equation sigma = alpha(ebeta epsilon -...

The optical and electronic properties of the {\alpha}-SiTe, {\beta}-SiTe, RX-SiTe_2 are investigated. A detailed analysis is done using standard density functional theory (DFT) hybrid (HSE06) methods. dielectric studied under three different methods: DFT, many-body Green's functions (GW), Bethe-Salpeter equation (BSE). Our calculations show that SiTe compounds possess extremely high static cons...