Spring frost is an important environmental stress that threatens the production of Prunus trees. However, little information is available regarding molecular response of these plants to the frost stress. Using high throughput sequencing, this study was conducted to identify differentially expressed miRNAs, both the conserved and the non-conserved ones, in the reproductive tissues of almond tole...

The activity of Prunus dulcis (sweet almond) β-glucosidase at the expense of p-nitrophenyl-β-d-glucopyranoside at pH 6 was determined, both under steady-state and pre-steady-state conditions. Using crude enzyme preparations, competitive inhibition by 1-5 mM imidazole was observed under both kinetic conditions tested. However, when imidazole was added to reaction mixtures at 0.125-0.250 mM, we d...

BACKGROUND The identity of allergenic almond proteins is incomplete. OBJECTIVE Our objective was to characterize patient IgE reactivity to a recombinant and corresponding native almond allergen. METHODS An almond cDNA library was screened with sera from patients with allergy for IgE binding proteins. Two reactive clones were sequenced, and 1 was expressed. The expressed recombinant allergen...

Random amplified microsatellite polymorphism (RAMP) is a PCR-based marker which uses a combination of two classes of markers: Simple sequence repeat (SSR) and Random amplified DNA polymorphism (RAPD) markers. RAMP has been demonstrated to be a potentially valuable molecular marker for the study of genetic relationships in cultivated plant species. The objective of this study was to optimize the...

Intensive livestock management impacts forest and trees in different ways. Pig manure is a major source of nitrogen (N) pollution surface ground waters some European regions such as north-eastern Spain, but it understudied how application agroforestry systems. How pig affects tree radial growth the N cycle was assessed by measuring concentrations soil, leaves wood δ15N tree-ring two species wid...

Drought stress is the most important factor limiting successful production of agricultural crops worldwide. The response some almond cultivars grafted on GF677 rootstock was studied to drought at a research greenhouse in Horticultural Science Research Institute Karaj, Iran, 2016-2017 growing season. plant materials included 12 (Prunus dulcis Mill.) genotypes/cultivars, including ‘Sh10’, ‘Saba’,...