Recently, it has been reported that small interfering RNAs can silence mutant alleles of genes while not affecting wild-type alleles. This high specificity should also limit off-target effects when these molecules are used in drug-target validation or as therapeutics. Small RNA technology is both extremely versatile, because virtually any expressed gene can be inhibited, and effective, because ...

introduction systemic lupus erythematosus (sle) is a complex autoimmune and inflammatory disease. many studies show hla alleles can be associated with sle. the aim of this study was to determine the association of hla-drb1 alleles with juvenile- onset in iranian children. materials and methods at a case – control study, 31 children with systemic lupus erythematosus (case group) who referred to ...

in this study, 110 samples of bread wheat, aegilops crassa, ae. cylindrica and ae. tauschii were investigated to identify low-molecular-weight glutenin subunits. overall, the four alleles in term of the analysis of sds-page gels were detected. the ʻaʼ allele with 45% has the greatest frequency, and after that, ʻbʼ, ʻcʼ and ʻdʼ alleles were with 38.1%, 11.8% and 9.1% frequencies, respectively; h...

Abstract Researchers have hypothesized that wild stingless bee colonies are a repository of genes for managed populations via the mating virgin queens with males from colonies. We tested this hypothesis bee, Scaptotrigona mexicana , culturally important species in study region. Each ten were split into two and placed meliponary, which resulted queen-right mother queenless daughter allowed to pr...

genetic diversity is one of the three levels of biodiversity. the aim of present study was to compare levels of genetic polymorphism between wild bream populations using seven microsatellite loci. genetic diversity was investigated by studying samples collected from two regions, the coast of chamkhale and bandaranzali of gilan province. a total of seven microsatellite loci (mfw7, mfw26, mcs1eh,...

the aim of this study was to investigate the association of bovine leukocyte antigen-drb3 alleles with peripheral blood mononuclear cell (pbmc) proliferation in response to staphylococcus aureus. the animals included in this study (n=347) were approximately of same age and comprised of f2 holstein-friesian ´ charolais (n = 155), holstein-friesian backcross (f0 holstein-friesian dams crossed wit...

Background: HLA compatibility between transplant donor and recipient is one of the major determinants of transplant outcome. Objective: To determine HLA class I by PCR- Sequence-Specific Oligonucleotide Probe (PCR-SSOP) in cord blood donors. Methods: Genomic DNA of 142 cord blood samples registered at the Cord Blood Bank of Iran at Hematology, Oncology, and Bone Marrow Transplantation Researc...

Background: Different alleles of Fragile X Mental Retardation1 (FMR1) gene with separate molecular etiologies cause Fragile X Syndrome (FXS) and Fragile X-associated Tremor and Ataxia Syndrome (FXTAS). Premutation alleles with 55 to 200 repeats in the FMR1 gene lead to FXTAS. It is carried by 1 in 209 women and 1 in 430 men. FXTAS commonly appears in 50- to 70-year-old adults. Case Presentatio...

almonds are self incompatible, the incompatibility being controlled by a multi allelic (s-alleles) single gene. the incompatibility alleles were studied in 96 samples of wild almonds and related prunus species from 10 taxonomic groups. polymerase chain reactions were carried out with six primer sets including: three degenerate primer pairs (paconsi-f(fam)/em-pc1consrd, paconsi-f(fam)/em-pc3cons...