نتایج جستجو برای: all loci were polymorphic

تعداد نتایج: 4459704  

G. Rahimi Mianji, M. Afzali, M. Gholizadeh,

In the present study we evaluated the amount and distribution of genetic variation by using RAPD marker variation of 12 markers loci in three broodstock groups of rainbow trout. A total of 47 polymorphic bands were observed in Iranian strain, average number of bands was 10 and average number of polymorphic bands per primer was 3.92. The total detected bands in rainbow trout strain originated fr...

2013
Na Wei Christopher W. Dick Andrew J. Lowe Michael G. Gardner

PREMISE OF THE STUDY Polymorphic microsatellite loci were characterized in the dioecious neotropical rainforest tree Virola sebifera. The markers will be used to study ecological and genetic impacts of hunting and landscape change in this vertebrate-dispersed, insect-pollinated tree species. METHODS AND RESULTS Simple sequence repeat (SSR) markers were screened from genomic libraries of South...

Journal: :Molecular ecology resources 2008
Phillip A Wadl Xinwang Wang Brian E Scheffler Timothy A Rinehart Robert N Trigiano

Microsatellite loci were identified from Cornus kousa'National'. Primer pairs for 86 loci were developed and of these, eight were optimized and screened using genomic DNA from 22 kousa cultivars. All optimized loci were polymorphic and the number of alleles per locus ranged from three to 17. Observed heterozygosity ranged from 0 to 0.3 and expected heterozygosity ranged from 0.38 to 0.91. These...

2016
Lu-Shui Zhang Lu Tan Dai-Mei Hu Yan Chen

PREMISE OF THE STUDY Polymorphic microsatellite markers were developed to facilitate studies on the fine-scale population genetic structure of Ficus tikoua (Moraceae), a prostrate shrub known to have highly restricted gene flow. METHODS AND RESULTS Microsatellite primers were developed using the biotin-streptavidin capture method and scanned for polymorphism within 76 individuals sampled from...

Journal: :Molecular ecology resources 2009
H Hürner J F Martin A Ribas A Arrizabalaga J R Michaux

We isolated and characterized 10 dinucleotide microsatellite loci in the edible dormouse, Glis glis (Linnaeus). Four multiplex panels were developed. Loci were amplified in samples from two geographically distant populations (Torgny in Belgium and Montseny in Spain). All loci were polymorphic in Spain but four were monomorphic in Belgium. Individuals from Belgium and Spain exhibited an average ...

2013
Ting Liu Seping Dai Wei Wu Rongshu Zhang Qiang Fan Suhua Shi Renchao Zhou

UNLABELLED PREMISE OF THE STUDY Microsatellite markers were developed for Melastoma dodecandrum to investigate the genetic diversity of this species and to detect hybridization and introgression in Melastoma. • METHODS AND RESULTS Fourteen microsatellite loci were characterized by screening primers developed using two simple sequence repeat (SSR)-enriched libraries. Based on the genotyping...

Journal: :Genetics and molecular research : GMR 2014
A D Lopes C A Scapim C A Mangolin M F P S Machado

The purpose of this study was to analyze the genetic diversity of 15 sugary-1 sweet corn lines by microsatellite markers. One hundred pairs of simple sequence repeat primers that were mapped for field corn were tested. Of these primers, 15% were polymorphic, and all were selected for the evaluation. These primers identified a total of 39 alleles among the 15 loci that were evaluated. The number...

2016
Agathe Pirog Sébastien Jaquemet Antonin Blaison Marc Soria Hélène Magalon

The tiger shark Galeocerdo cuvier (Carcharhinidae) is a large elasmobranch suspected to have, as other apex predators, a keystone function in marine ecosystems and is currently considered Near Threatened (Red list IUCN). Knowledge on its ecology, which is crucial to design proper conservation and management plans, is very scarce. Here we describe the isolation of eight polymorphic microsatellit...

Evaluation of genetic diversity is the key principal for plant breeding, providing an opportunity to discover novel characters and alleles for breeders. In the present study, 69 durum wheat genotypes were investigated for genetic diversity using several inter-simple sequence repeat (ISSR) markers. Sixteen ISSR primers amplified a total of 163 fragments, which out 160 fragments were polymorphic....

Journal: :Molecular ecology resources 2009
Blas Lavandero Christian C Figueroa Claudio C Ramirez Peter D S Caligari Eduardo Fuentes-Contreras

Eight novel microsatellite primer pairs are presented for Eriosoma lanigerum, representing the first microsatellite markers available for this genus. Loci were characterized for 27 individuals from one single orchard in Central Chile. All loci were polymorphic within E. lanigerum (three to 11 alleles per locus; observed heterozygosity ranging from 0.41 to 0.93), and are therefore useful for pop...

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