RNAi is a gene-silencing phenomenon triggered by double-stranded (ds) RNA and involves the generation of 21 to 26 nt RNA segments that guide mRNA destruction. In Caenorhabditis elegans, lin-4 and let-7 encode small temporal RNAs (stRNAs) of 22 nt that regulate stage-specific development. Here we show that inactivation of genes related to RNAi pathway genes, a homolog of Drosophila Dicer (dcr-1)...

Polyethyleneimine (PEI)-alginate (Alg) nanoparticle (NP) is a safe and effective vector for delivery of siRNA or DNA. Recent studies suggest that autophagy is related to cytotoxicity of PEI NPs. However, contribution of autophagy to degradation of PEI-Alg NPs remains unknown. CD34+VEGFR-3+ endothelial progenitor cells isolated from rat bone marrow were treated with 25 kDa branched PEI modified ...

ALG-2, a 22-kDa penta-EF-hand protein, is involved in cell death, signal transduction, membrane trafficking, etc., by interacting with various proteins in mammalian cells in a Ca2+-dependent manner. Most known ALG-2-interacting proteins contain proline-rich regions in which either PPYPXnYP (type 1 motif) or PXPGF (type 2 motif) is commonly found. Previous X-ray crystal structural analysis of th...

Objective In the present study, the effects of alginate (ALG) concentration and ovarian cells (OCs) on the development and function of follicles were simultaneously evaluated. MaterialsAndMethods In the first step, preantral follicles (100-130 µm in diameter) were mechanically isolated from the ovaries of 2-week-old NMRI mice, encapsulated in the absence or presence of OCs in 0.5, 0.75 and 1% A...

Intonational phrasing and pitch accent distribution (PAD) have been proposed to be interdependent (Portuguese) and independent properties of prosodic systems (Egyptian). This paper examines the relation between intonational phrasing and pitch accent distribution in two center-southern varieties of European Portuguese Alentejo (Ale) and Algarve (Alg). Sentences obtained in a reading task systema...

Clinical reactions and serologic in all cases, to alpha globulins in 9 cases, and to SuMM*~y changes after intramuscular adgamma globulins in only 1. This h d i n g indicates ministration of horse anti-human lymphocyte that a safer ALG could be made by removing the globulin (ALG) were studied in 53 human retrace quantities of alpha and beta globulins from cipients of renal homografts. The ALG w...

We report the spatiotemporal control of a 3D coculture by combining photocurable and chemically degradable hydrogels for coculture of embryonic stem (ES) cells with HepG2 cells. ES cells were encapsulated in micropatterned photocurable poly(ethylene glycol) (PEG) hydrogels. The cell-laden PEG hydrogels were then encapsulated in calcium-alginate (Ca-Alg) hydrogel containing HepG2 cells. The ES c...

Then, f ðR ijk;Ri;Rj;RkÞ is either faj; alg; faj; akg or faj; ak; alg: (Only the first two possibilities are mentioned on page 390). In Case A, also consider the possibility that f ðR ijk;Ri;Rj ;RkÞ 1⁄4 faj; ak; alg: Choose RkADE such that falgPkfaj; algPkfajgPkfak; al ; algPkX for any other set X : The proof that k will manipulate at ðR ijk;Ri; % Rj ;RkÞ via Rk is virtually the same as before....