BACKGROUND In Japan, epizootic arboviral infections have severely impacted the livestock industry for a long period. Akabane, Aino, Chuzan, bovine ephemeral fever and Ibaraki viruses have repeatedly caused epizootic abnormal births and febrile illness in the cattle population. In addition, Peaton, Sathuperi, Shamonda and D'Aguilar viruses and epizootic hemorrhagic virus serotype 7 have recently...

UNLABELLED We generated a recombinant Akabane virus (AKAV) expressing enhanced green fluorescence protein (eGFP-AKAV) by using reverse genetics. We artificially constructed an ambisense AKAV S genome encoding N/NSs on the negative-sense strand, and eGFP on the positive-sense strand with an intergenic region (IGR) derived from the Rift Valley fever virus (RVFV) S genome. The recombinant virus ex...

Technology for virus multiplication has been greatly advanced since the establishments of the chick embryo culture method^ and tissue culture method.2) These methods have contributed to progress in virology and vaccine preparation. Many vaccines for virus diseases of human and domestic animals have been developed after Salk's success in preparing poliovaccine using primary culture of monkey kid...

BACKGROUND Schmallenberg virus (SBV), Akabane virus (AKAV) and Aino virus (AINV) are members of the Simbu serogroup within the genus Orthobunyavirus, family Bunyaviridae, which can cause reproductive disorders including abortion, stillbirth and congenital malformation in ruminants. Because, the clinical signs are similar, confirmatory diagnosis requires viral detection to differentiate infectio...

Rift Valley fever is the most important bunyaviral disease of animals in Africa. The virus, transmitted by mosquitoes, causes abortions and mortality in young animals in addition to haemorrhagic fevers in humans. Although vaccines against this virus are available, the uses of these vaccines are limited because of deleterious effects or incomplete protection, justifying further studies to improv...

Rift Valley fever virus (RVFV) is one of the important emerging viral diseases of serious impact in public health and animal hygiene both in human and animal industries. In this study, we developed a monoclonal antibody-based competitive ELISA for the detection of antibodies to RVFV in goats and cattle. The recombinant N protein of RVFV was expressed in E. coli with a six-histidine tag, and the...

Hisakazu Kikuchi, Hiroto Sato, Satoshi Hasebe, Naoki Mizutani, Shogo Muramatsu, Shigenobu Sasaki, Zie Zhou, Seishi Sekine, Yoshito Abe , and Makoto Nakashizuka 2 Department of Electrical and Electronic Engineering, Niigata University Niigata 950-2181, Japan 1 Integrated Manufacturing Technology Laboratory, Dai Nippon Printing Co., Ltd. 2-3-6 Akabane Minami, Kita-ku, Tokyo 115-8611, Japan 2 Grad...

Akabane (AKA) virus is an arthropod-borne virus belonging to the Simbu group of the genus Bunyavirus. Neutralizing monoclonal antibodies (MAbs) against AKA virus were prepared, and the neutralizing epitopes of the virus were defined by competitive binding assay. Five distinct antigenic domains were identified and were designated A, B, C, D, and E. Domains A and C consisted of two epitopes each....