Protoplasts represent a unique experimental system for the circulation and formation of genetically modified plants. Here, protoplasts were isolated from hairy root tissues Brassica oleracea L. induced by Agrobacterium rhizogenes strain (ATCC13332). The concentration enzyme solutions utilized protoplast isolation was 1.5 % Cellulase YC 0.1 Pectolyase Y23 in 13% mannitol solution, which resulted...

Hairy root culture (HRC) represent a valuable biotechnological tool for the production of plant secondary metabolites. Secondary metabolome study Arabidopsis thaliana may help to understand biological roles various metabolites present in it. The work deals with establishment Agrobacterium rhizogenes strains transformed HRC A. very high transformation frequency resulting long term hairy cultures...

The complete soybean leghemoglobin lbc(3) gene was transferred into the legume Lotus corniculatus using an Agrobacterium rhizogenes vector system. Organ-specific expression of the soybean gene was observed in root nodules formed on regenerated transgenic plants after infection with Rhizobium loti. The primary transcript was processed in the same way as in soybean nodules and the resulting mRNA ...

Analysis of genes preferentially expressed in hairy roots caused by infection with Agrobacterium rhizogenes has provided insights into the regulation of lateral root formation. A hairy root preferential cDNA, HR7, has been cloned from hairy roots of Hyoscyamus niger. HR7 encodes a novel protein partially homologous to a metallocarboxypeptidase inhibitor and is expressed exclusively in the primo...