The isolation of cell surface mutants of Acinetobacter calcoaceticus RAG-1 was demonstrated by a method based on the differential immunoprecipitating properties of wild-type cells and mutants. In the presence of antiserum, about 99% of wild-type cells in suspension sedimented following overnight agglutination. The kinetics of antiserum-induced agglutination was quantified by measuring the time-...

Staphylococcus aureus is a prominent bacterial pathogen that is known to agglutinate in the presence of human plasma to form stable clumps. There is increasing evidence that agglutination aids S. aureus pathogenesis, but the mechanisms of this process remain to be fully elucidated. To better define this process, we developed both tube based and flow cytometry methods to monitor clumping in the ...

Intact human erythrocytes were cross-linked with glutaraldehyde (GA) or dimethyladipimidate (DMA) and tested for their ability to bind [125I]-IgG anti-Rh0(D) and to undergo antibody-mediated hemagglutination. There was no decrease in antibody binding after treatment with GA concentrations up to 1.25% and DMA concentrations up to 1%. Red cells treated with these concentrations of GA and DMA did ...

This study concerns the preparation of reagents for identifying the somatic O antigens of Salmonella enteritidis. Coagglutination reagents (COAGs) with antibody fixed to killed and stabilized protein A-bearing staphylococci were prepared with antisera which were used for identifying the somatic O antigens of S. enteritidis by the slide agglutination test. The reactions of the COAGs were compare...

cryptococcosis has become the fourth leading life-threatening opportunistic infection in patients with aids, but also occurs in non-aids patients. in view of the increasing numbers of infection during last decade in iran, use of rapid, sensitive and specific test for diagnosis of cryptococcal disease has become important than ever. we aimed to produce the reagents for latex cryptococcal antigen...

background because of the economic importance of the arab race horses and also the role of toxoplasma gondii and neospora spp. in abortion and reproductive failure of these animals, we decided to perform this study. objectives we designed this study to investigate the seroprevalence of anti-toxoplasma gondii and anti-neospora spp. antibodies in arab horses from 12 cities of khuzestan province i...

Lipopolysaccharide (LPS) is a major surface component of most Gram-negative bacteria and recognized by immune cells as pathogen-associated molecules. Antibodies against O antigens can provide protection infection vaccines have been developed conjugating to carrier proteins enhance immunogenicity. This study aims produce antigen from Escherichia coli analyze the in vivo test results poultry usin...

In this study a device was designed to detect human blood group using the ABO method which is integrated with oxygen saturation meter. This tool can agglutination or non-agglutination reactions in samples that have been treated antisera and will then be read by three TCRT5000 sensors are reflective used as detectors of A, B Rhesus antigens when occur on paper. sample test. addition, MAX30100 se...

One hundred sera from 49 patients with glandular toxoplasmosis were examined by a latex agglutination test, the dye test, an indirect haemagglutination test, and a double antibody sandwich enzyme linked immunosorbent assay (ELISA) for antitoxoplasma IgM. The results support previous findings that the dye test, indirect haemagglutination test, and latex agglutination test measure different antib...

The association between occupancy of the von Willebrand factor (vWf) receptor glycoprotein (GP) Ib, agglutination, and the assembly and composition of the cytoskeletal core was studied in 125I-surface-labeled aspirin-treated washed platelets. Binding of ligands to GPIIb-IIIa and platelet aggregation were abolished by addition of EDTA. Platelet agglutination induced by bovine vWf generated a com...