The aim of this study was to construct, expression of a novel recombinant chimeric protein consisting of Pyruvate dehydrogenase beta subunit (PDHB) and high antigenic region of integral membrane lipoprotein P80 of Mycoplasma agalactiae as a potential diagnostic tool. The full-length sequence of pdhb and a portion of antigenic regions of P80 were selected and analyzed by CLC ma...

© 2005, Pamela L. Ruegg Premiums, Production and Pails of Discarded Milk How Much Money Does Mastitis Cost You? Pamela Ruegg, DVM, MPVM University of Wisconsin, Madison Introduction Profit centered dairy farms strive to maximize milk price and control costs. One way to control costs is by minimizing the rate of disease. The most costly disease of dairy cattle is generally considered to be masti...

We developed a new recombinant enzyme-linked immunosorbent assay (rELISA) for serodiagnosis of contagious agalactia (CA), a disease caused by Mycoplasma agalactiae in sheep and goats. The assay is based on two M. agalactiae surface proteins, namely, P80 and P55. Identification of these immunodominant and common antigens was accomplished by examining the antibody response elicited in sheep durin...

BACKGROUND Laboratory diagnostic techniques able to detect Mycoplasma agalactiae are essential in contagious agalactia in dairy goats. This study was designed: 1) to determine the detection limits of PCR and culture in goat milk samples, 2) to examine the effects of experimental conditions including the DNA extraction method, PCR technique and storage conditions (fresh versus frozen stored milk...

1. Flunixin is a non-steroidal anti-inflammatory drug (NSAID), and a non-narcotic analgesic with antipyretic activities. In veterinary medicine, it is used with meglumine as solubilizer as flunixin meglumine. Flunixin meglumine is used in the alleviation of inflammation and pain associated with musculo-skeletal disorders and colic in horses; the control of acute inflammation associated with inf...

Mycoplasma agalactiae and Mycoplasma bovis both are responsible for respiratory conditions in sheep and goats. M. agalactiae is a major pathogen of sheep and goats and accounts for almost 90% of outbreaks of contagious agalactia syndrome in goats and almost 100% in sheep. On the basis of clinical signs and cultural, morphological, and biochemical characterization it is almost impossible to diff...

A 36-year-old woman with postpartum hypopituitarism (Sheehan's syndrome: SS) developed postpartum autoimmune thyroiditis (PPAT). She delivered a baby by Caesarean section (620 mL blood loss). At 1 month post partum, she developed thyrotoxicosis due to painless thyroiditis (autoimmune destructive thyroiditis). She was positive for antithyroid antibodies. Postpartum and hypoadrenalism-induced exa...