in this study one lymph node from cow with viral lymphosarcoma and one lymph node from healthy cow were analyzed by three different methods for protein extraction, include simple homogenizing in pbs, sonication and phenol extraction. tumor antigen detection relies on immunoprecipitation followed by sds-page. in this experiment streptococcus pyogenes was used for purification of immune complexes...

Hydatidosis is a disease caused by infection with the metacestode stage of the dog tapeworm,Echinococcus granulosus. This is recognised as one of the world’s most important zoonoses, affecting bothhumans and their domestic animals. In the current study, 20 sheep cystic livers or lungs were collected.Hydatid fluid and protoscolices were isolated and the soluble protein was prepared. Polypeptide ...

background: cutaneous leishmaniasis (cl) is a parasitic disease with a relatively wide distribution in different areas of the world, including iran. the parasite is mainly diagnosed microscopically, but serological approaches might be useful for diagnosis as well.  this study aimed to assess the efficacy of an immunoblotting system for serodiagnosis of cutaneous leishmaniasis in iran. methods: ...

Humic substances (HS) are the most important natural biostimulant of plants. However, relationship between their structure and biological activity in plants is still not well recognized. The objective this paper was to assess influence molecular fractions humic acids (HA) (HA < 30 kDa HA > kDa) on reducing negative effects drought stress soybean (Glycine max (L.) Merr.) seedlings Progres ...

Abstract Dissolved organic matter (DOM) concentrations have been increasing in parts of the northern hemisphere for several decades. This process—brownification—often accompanies iron and aluminum, but metal–DOM interactions these concurrent trends imply are poorly described. Here we used field-flow fractionation with UV ICP-MS detection to measure size distribution colloidal iron, manganese, c...

Abstract. Rahayu SYS, Fadila A, Fahmi MR. 2023. Identification of metallothionein protein in Anodonta woodiana as a biomarker mercury (Hg) contamination. Nusantara Bioscience 15: 90-94. Heavy metal contamination can affect the survival aquatic biota and will accumulate bodiesorganisms. Moreover, identification at molecular level be analyzed using analysis. Biomarkers are responses measured indi...

Background: Some antigens of H. pylori are excreted into the stool of infected people. These antigens can be used to detect the infection by immunoassays such as ELISA. Our aim was to identify these antigens by immunoblotting and affinity chromatography techniques. Methods: Four different antigenic preparations, namely, whole cell sonicate (WCS), outer membrane proteins (OMPs), cytoplasmic anti...

Membrane diafiltration process was developed in order to fractionate the water soluble proteins of supernatant obtained after Spirulina bead milling disruption and centrifugation. The crude extract filtered with 50 kDa, 150 300 kDa or 0.2 µm membranes. Phycocyanin purified extracts were produced filtration on membrane allowed high recovery (more than 89%) A C into retentate permeate as well sep...