introduction: a noninvasive method of detecting exposure of phosphatidylserine (ps) on the external surface of the plasma membrane such as nuclear imaging could assist the diagnosis and therapy of apoptosis related pathologies. the most studied imaging agent for apoptosis is annexin v so far. because of limitations of annexin v other agents have been introduced such as small peptides and molecu...

Identification of components present in biological complexes requires their purification to near homogeneity. Methods of purification vary from protein to protein, making it impossible to design a general purification strategy valid for all cases. We have developed the tandem affinity purification (TAP) method as a tool that allows rapid purification under native conditions of complexes, even w...

Market basket analysis (MBA) is a widely used technique for identifying affinities among items that customers purchase together. MBA metrics are support, confidence, and lift. We show that support and confidence may include misleading information about the nature of the affinity, and that lift is the most useful metric. Starting with the MBA, we use the product affinities to predict ways to inc...

A single-step protein affinity purification protocol using Aspergillus nidulans is described. Detailed protocols for cell breakage, affinity purification, and depending on the application, methods for protein release from affinity beads are provided. Examples defining the utility of the approaches, which should be widely applicable, are included.

To learn the molecular mechanism of an enzyme action, first the protein has to be purified. Because of its simplicity and reproducibility, affinity chromatography has become a adopted method throughout the scientific community. Affinity chromatography occupies a unique place in separation technology since it is the only technique which enables purification of almost any biomolecules on the basi...

Acetylation signaling pathways are involved in numerous cellular processes and are used as therapeutic targets in several disease contexts. However, acetylated proteins only represent a minor fraction of the full proteome, and the identification and quantification of acetylated sites remain a technological challenge. Currently, pan-acetyl antibodies are used to increase the abundance of acetyla...

We suggest structure characterization of macromolecular assemblies by combining assembly shape determined by electron cyromicroscopy with information about subunit proximity determined by affinity purification. To achieve this aim, structure characterization is expressed as a problem in satisfaction of spatial restraints that (1) represents subunits as spheres, (2) encodes information about the...

The taxonomic affinity of six species of Crocus (C, cancellatus subsp. Damascenus C. almehensis, C. gilanicus, C. sativus, C.pallassi subsp. haussknechtii,C. speciosus subsp. speciosus) from Iran were studied usingdifferent electrophoresis techniques. The similarity index of the species was computed by comparison of protein bands of - Corm and seed tissues, and the related dendrogms of affi...