The adrenergic amines noradrenaline and adrenaline increased flux through phenylalanine hydroxylase by approx. 50%. This effect, which appears to be mediated by an alpha-adrenergic mechanism, was accompanied by a rapid increase in the phosphorylation of phenylalanine hydroxylase. Although ionophore A23187 mimicked the effects of the adrenergic amines, vasopressin was completely without effect o...

Although quinidine is known to have antiadrenergic effects in the cardiovascular system, the precise mechanism by which it exerts these effects is not well defined. We asked whether quinidine binds directly to adrenergic receptors. Radioligand-binding assays were used to identify <*i -adrenergic receptors ([H]prazosin-binding sites) on membranes prepared from rat heart and kidney, a2-adrenergic...

A procedure has been developed for purification of the porcine brain alpha 2-adrenergic receptor to homogeneity. alpha 2-Adrenergic receptors were solubilized from porcine brain particulate preparations using sequential extraction into sodium cholate- and digitonin-containing buffers. The alpha 2-adrenergic receptors in the digitonin extract were identified using the alpha 2-adrenergic selectiv...

The aim of the present study was to examine gene expression and protein concentrations of beta(1)- and beta(2)-adrenergic receptors in subcutaneous adipose tissue in obese subjects in response to weight loss. Eighteen obese subjects were studied during diet-induced weight loss. Beta-adrenergic receptor mRNA levels were quantified by reverse transcription-PCR-HPLC. Beta-adrenergic receptor prote...

In 1958, Powell and Slater (1) first described that DCI had beta-adrenergic blocking activity even though it included beta-adrenergic stimulations. Such property was found also in methoxamine besides its alpha-adrenergic stimulant activity (2, 3). In the follow ing decade, many beta-adrenergic blocking agents have been synthesized successively (4-8) and one of them, propranolol was first introd...

The technique of quantitative autoradiography has been used to localize changes in the densities of subtypes of beta-adrenergic receptors in rat brain following treatment with 6-hydroxydopamine. Previously reported increases in the density of beta 1-adrenergic receptors in the cerebral cortex were confirmed. The anatomical resolution of autoradiography made it possible to detect changes in the ...

introduction: malignant astrocytic gliomas are the most common and lethal brain malignancies due to their refractory to the current therapies. nowadays, molecular targeted therapy has attracted great attention in treatment of glioma. connexin 43 (cx43) and micro ribonucleic acid- 21(mir-21) are among molecules that are involved in glioma development and progression. these molecules showed poten...

The effects of epidermal growth factor (EGF) on the beta-adrenergic receptor-coupled adenylyl cyclase system were studied in a human salivary cell line (HSY). The beta-adrenergic agonist isoproterenol (10(-5) M) stimulated adenylyl cyclase activity by approximately 2-fold, and the isoproterenol response was increased 1.8-fold after prolonged (48 h) exposure to EGF (5 x 10(-10) M). In contrast, ...

The presence of adrenergic axons within the sphincter of Oddi of the cat and dog was demonstrated using the glyoxylic acid-induced fluorescence histochemical method. After vagotomy (in cats), no reduction was observed in the number of the adrenergic axons or in the fluorescence intensity. It is concluded that the sphincter of Oddi receives direct adrenergic neural control derived from the sympa...