While the interactions of cells with polymeric substrata are widely studied, the influence of cell-cell cohesiveness on tissue spreading has not been rigorously investigated. Here we demonstrate that the rate of tissue spreading over a two-dimensional substratum reflects a competition or "tug-of-war" between cell-cell and cell-substratum adhesions. We have generated both a "library" of structur...

To establish liver tissue engineering, the effective substratum for hepatocytes culture should be developed. Up to now, it is believed that Matrigel, which contains several basement membrane proteins produced by sarcoma cells, is the most effective substratum. Matrigel does not contain extracellular matrix molecules derived from epithelial cells although the space of Disse contains the molecule...

objective: in all protocols for isolation of mesenchymal stem cells (mscs), a few days after culture initiation, the medium were discarded along with its contents of non-adherent cells and the adherent cell population kept and expanded as mscs population. in the present study, attempt was made to expand the cells suspended in removed medium of primary culture and compare them with the adherent ...

In this study, the distribution of channel-bed sediment facies in the lowermost Mississippi River is analysed using multibeam data, complemented by sidescan sonar and compressed high-intensity radar pulse seismic data, as well as grab and core samples of bed material. The channel bed is composed of a discontinuous layer of alluvial sediment and a relict substratum that is exposed on the channel...

Previous studies in the wolf spider Lycosa tarantula (Linnaeus 1758) have shown that homing is carried out by path integration and that, in the absence of information relative to the sun's position or any pattern of polarized light, L. tarantula obtains information as to the angle it must turn to home through the anterior lateral eyes (ALEs). In the present study, spiders were trained to walk o...

We analysed the spatial patterns in macroinvertebrate taxon richness and abundance at two scales: sampling unit and basin. We sampled 12 stream sites in three zones of Portugal, differing in climate geomorphology and water chemistry. At a sampling unit scale, substratum organic matter content, depth and the dominant size of substratum particles were correlated with numbers of taxa and individua...

A heuristic way to calculate the approximate value of the Casimir force is introduced

introduction: while human endothelial progenitor cells (epcs) have been a subject of somehow extensive investigation, epcs from adult mouse hematopoietic system were poorly studied. present investigation is focused on fvb mouse endothelial progenitor cells in terms of their isolation, purification, and expansion. materials and methods: mononuclear cells collected from murine peripheral blood we...

The box tropical mussel Septifer bilocularis lives attached to a hard substratum, forming dense aggregation or patch. occurs at the time of larvae settlement in response different cues. Larvae may have positive whereas settle and attach while negative response, postpone settling occurs. Byssus threads are produced for some reason, they affected by many variables. We studied whether attachment w...