a total of 1695 blood samples collected from ten ethnically distinct populations of iran (turks and kurds of rezaieh, lurs, aabolis, baluchis, turks and kurds of shrvan, zoroastrians, tehranis, and kermanis) were examined for six polymorphic red cell enzyme systems, namely red cell acid phosphatase (ap), adenylate kinase (ak), phosphoglucomutase (pgm1), esteraxe d (esd), adenosine deaminase (ad...

V. A. KNIVETT I954 3. [32P]Potassium dihydrogen phosphate was used to distinguish between ATP formed from ADP by the action of adenylate kinase (myokinase) and by synthesis involving inorganic phosphate. The appearance of 32P in ADP, and in both of the labile phosphates of ATP, but not in AMP, indicates the presence of adenylate kinase in extracts of Strep. faecali&. 4. The fission of citrullin...

Adenylate kinases (AKs) play important roles in nucleotide metabolism in all organisms and in cellular energetics by means of phosphotransfer networks in eukaryotes. The crystal structure of a human AK named AK6 was determined by in-house sulfur single-wavelength anomalous dispersion phasing methods and refined to 2.0-A resolution with a free R factor of 21.8%. Sequence analyses revealed that h...

Intact mitochondria, obtained from Euglena gracilis Klebs var. bacillaris Cori mutant W10BSmL, which lacks plastids, and purified on Percoll density gradients, form adenosine 3'-phosphate 5'-phosphosulphate from sulphate. The optimal conditions include addition of 17 mM-Tricine/KOH, pH 7.6, 18 mM-MgCl2, 250 mM-sucrose, 5.66 mM-sodium ADP (or 0.94 mM-sodium ATP), 1 mM-K2SO4, carrier-free 35SO4(2...

Correlated inter-domain motions in proteins can mediate fundamental biochemical processes such as signal transduction and allostery. Here we characterize at structural level the inter-domain coupling in a multidomain enzyme, Adenylate Kinase (AK), using computational methods that exploit the shape information encoded in residual dipolar couplings (RDCs) measured under steric alignment by nuclea...

Experiments were carried out to clarify the sites of action of beta-adrenergic agonists in skeletal muscle microsomes. Microsomes were fractionated into longitudinal reticulum, terminal cisternae, and isolated transverse tubules. Transverse tubules were selectively labeled and tracked with [3H]ouabain. beta-adrenergic receptor was identified by [3H]dihydroalprenolol binding. Assays of beta-adre...

The current methods for the determination of creatine kinase (EC 2.7.3.2) activity are derived from Oliver's method, in which AMP is used to decrease interference by adenylate kinase (EC 2.7.4.3). Recently, Szasz et al. and Rosano et al. described methods in which diadenosine pentaphosphate and fluoride, respectively, are used to reduce this interference. However, diadenosine pentaphosphate doe...

Muscarinic acetylcholine receptors exert slow and prolonged synaptic effects in both vertebrate and invertebrate nervous systems. Through activation of G proteins, they typically decrease intracellular cAMP levels by inhibition of adenylate cyclase or stimulate phospholipase C and the turnover of inositol phosphates. In insects, muscarinic receptors have been credited with two main functions: i...

Tumor growth and the induction of adenylate kinase (EC 2.7.4.3) and pyruvate kinase (EC 2.7.1.40) in transplantable rat hepatomas were studied in organ-ablated and hormonally treated rats that were fed different diets. Tumor growth was slowed when the rats were (a) fasted, (b) diabetic and fed chow, or (c) diabetic and fasted. Tumor growth increased in diabetic rats fed high-glucose diets or tr...

Activities of acetyl-C oA synthetase (E C 6 .2 .1 .1), adenylate kinase (E C 2 .7.4.3) and glycerate kinase (E C 2.7.1.31) w ere found in leaf extracts of iojap m utan t m aize (Zea mays) which lacks the protein-synthesizing apparatus in plastids. These activities rep resen ted at least one-half of the ra tes observed for norm al maize plants. Since the p ro teins stud ied are localized e ither...