When fragments of rat or human adipose tissue, or isolated adipocytes, are incubated with [14C]glucose in vitro, [14C]diacylglycerol accumulates rapidly: it comprises 20-50% of newly synthesized (14C-labeled) acylglycerols, compared to less than 1% diacylglycerol accumulated in the bulk lipid store in vivo. The experiments reported in this study were performed to test the possibility that agent...

Carboxyl ester lipase (CEL), previously named cholesterol esterase or bile salt-stimulated (or dependent) lipase, is a lipolytic enzyme capable of hydrolyzing cholesteryl esters, tri-, di-, and mono-acylglycerols, phospholipids, lysophospholipids, and ceramide. The active site catalytic triad of serine-histidine-aspartate is centrally located within the enzyme structure and is partially covered...

Modification of hypothalamic fatty acid (FA) metabolism can improve energy homeostasis and prevent hyperphagia and excessive weight gain in diet-induced obesity (DIO) from a diet high in saturated fatty acids. We have shown previously that C75, a stimulator of carnitine palmitoyl transferase-1 (CPT-1) and fatty acid oxidation (FAOx), exerts at least some of its hypophagic effects via neuronal m...

Human platelets convert [l-W]or [2J4C]acetate to labeled CO2 and fatty acids; the latter, in turn, become esterified to acylglycerols and phospholipids. However, acetate apparently does not reach mevalonate in the sterol pathway. By contrast, with [2J4C]mevalonate as substrate, there is labeling of farnesol, farnesoic acid, squalene, lanosterol, and dihydrolanosterol in the chloroform-soluble f...

Monoacylglycerols (MAGs) are structural and bioactive metabolites critical for biological function. Development of facile tools for measuring MAG are essential to understand its role in different diseases and various pathways. A data-independent acquisition method, MS/MS(ALL), using electrospray ionization (ESI) coupled quadrupole time of flight mass spectrometry (MS), was utilized for the stru...

Hormone-sensitive lipase, purified from rat adipose tissue (Fredrikson, G., Strålfors, P., Nilsson, N. O., and Belfrage, P. (1981) J. Biol. Chem. 256, 6311-6320), has been incubated with tri-, di-, and monooleoyl[3H]glycerol, and the acylglycerol reaction products were isolated by thin layer chromatography on silicic acid, impregnated with boric acid. Trioleoylglycerol was hydrolyzed with the i...

Lipoprotein lipase (EC 3.1.1.34), as the extrahepatic enzyme responsible for the hydrolysis of plasma lipoprotein triacylglycerol, plays a pivotal role in the metabolism of circulating lipids. The Occurrence and action of the enzyme in lipoprotein metabolism has been the subject of a number of recent reviews (Cryer, 1981 ; Quinn et al., 1982; Hamash & Hamash, 1983) and this aspect will not be c...

There is no uniformly adopted definition for lipids and it is widely accepted that lipids are fatty acids, their derivatives, and substances related biosynthetically or functionally to these compounds [1]. Glycerolipids –lipids based on glycerol (acylglycerols, phospholipids, glycolipids, etc.) –in living organisms are complex mixtures of different types of molecules that contain stereoisomers ...