Integron carriage by 36 epidemiologically unrelated Acinetobacter baumannii isolates collected over an 11-year period from patients in six different Italian hospitals was investigated. Sixteen type 1 integron-positive isolates (44%) were found, 13 of which carried the same array of cassettes, i.e., aacC1, orfX, orfX', and aadA1a. As ribotype analysis of the isolates demonstrated a notable genet...

Bioremediation of chromated copper arsenate-treated waste wood with one or more metal-tolerant bacteria is a potential method of naturally releasing metals from treated wood fibre. Sampling eight environments with elevated levels of copper, chromium, and arsenic resulted in the isolation of 28 bacteria with the capability of releasing one or more of the components from chromated copper arsenate...

A cross-reaction between Acinetobacter calcoaceticus and chlamydiae is described. A water-soluble, heat stable, non-dialyzable antigen was extracted from Acinetobacter species by boiling. This antigen fixed complement in the presence of homologous hyperimmune sera from rabbits or guinea pigs and in the presence of heterologous human or hyperimmunized animal sera containing chlamydial antibodies...

The in vitro activity of 18 antimicrobial agents against 40 clinical isolates each of Acinetobacter calcoaceticus subsp. anitratum and Acinetobacter lwoffi was studied. Most of the newer 4-quinolone derivatives were extremely active against these organisms. Newer beta-lactam agents, such as cefpirome, BMY 28142, and BRL 36650, were also extremely active, inhibiting all strains at clinically ach...

Acinetobacter baumannii is associated with hospital-acquired infections and can cause persistent outbreaks. Here we report the draft genome sequence of ABBL099, a multidrug-resistant clinical isolate of A. baumannii belonging to a novel sequence type and representative of clonal isolates cultured from patients at one institution over a 4-year time period.

Boric acid has been used for over 20 years to preserve urine while in transit for bacteriological examination. It has been suggested that it may be toxic for some urinary pathogens. To investigate this several strains of bacteria and fungi commonly found in urine were exposed to different concentrations of boric acid in nutrient broth. Viable counts were made at the outset and at intervals for ...

Glutathione transferases (GST) were purified from locally isolated bacteria, Acinetobacter calcoaceticus Y1, by glutathione-affinity chromatography and anion exchange, and their substrate specificities were investigated. SDS-polyacrylamide gel electrophoresis revealed that the purified GST resolved into a single band with a molecular weight (MW) of 23 kDa. 2-dimensional (2-D) gel electrophoresi...

Enrichment technique was employed for the isolation of the crude oil degrading bacteria. The isolated bacteria were screened for their degradative ability and the best degrading bacteria were selected based on their growth. Specific activities of Catechol-2,3-dioxygenase and effects of temperature and pH and their stabilities on the enzyme relative activities were observed. Bacteria isolated fr...